Reversed-phase high-performance liquid chromatographic separation of human phenolic metabolites of propoxur (Baygon), carbofuran and carbaryl
Keiser, JE; Kirby, KW; Tremmel, F
| HERO ID | 95118 |
|---|---|
| In Press | No |
| Year | 1983 |
| Title | Reversed-phase high-performance liquid chromatographic separation of human phenolic metabolites of propoxur (Baygon), carbofuran and carbaryl |
| Authors | Keiser, JE; Kirby, KW; Tremmel, F |
| Journal | Journal of Chromatography A |
| Volume | 259 |
| Issue | 1 |
| Page Numbers | 186-188 |
| Abstract | High performance liquid chromatography (HPLC) separation of human phenolic metabolites of the carbamate (302114) insecticides propoxur (114261), carbofuran (1563662), and carbaryl (63252) were investigated. The phenols examined were 2,3-dihydro-2,2-dimethyl-3-keto-7-hydroxybenzofuran, 2,3-dihydro-2,2-dimethyl-7-hydroxybenzofuran, 2-isopropoxyphenol (4812208), and 1-naphthol (90153). HPLC was performed on a reversed phase column. Solvent mixtures were tested. Two different gradient elutions using an acetonitrile/water solvent mixture were compared: 0 to 60 percent (10 minutes) and 30 to 60 percent (10 minutes). Several solvent mixtures were capable of separating the four phenolic metabolites on the reversed phase column including: 35 to 65 acetonitrile/water; 55 to 45 methanol/water; and 35 to 65 tetrahydrofuran/water. Peak 1 in the 0 to 60 percent, 10 minute, gradient was less efficient than the 30 to 60 percent gradient. With the 30 to 60 percent gradient the peaks also eluted in less than 4 minutes as compared to a 5 minute period in the 0 to 60 percent gradient. The authors conclude that the HPLC procedures enable separation of human urinary metabolites of three commonly used carbamate insecticides. |
| Doi | 10.1016/S0021-9673(01)87995-4 |
| Pmid | 6406526 |
| Wosid | WOS:A1983QK06800022 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020640593&doi=10.1016%2fS0021-9673%2801%2987995-4&partnerID=40&md5=a55b8f61a157287c7fa5a0a9ea4aa4fa |
| Is Public | Yes |
| Language Text | English |
| Keyword | 1 naphthol; carbaril; carbofuran; phenol; propoxur; 2,3 dihydro 7 hydroxy 2,2 dimethyl 3 benzofuranone; 2,3 dihydro 7 hydroxy 2,2 dimethylbenzofuran; carbamyl; drug analysis; drug determination; drug identification; drug metabolism; drug urine level; high performance liquid chromatography; human; isopropoxyphenol; methodology; reversed phase liquid chromatography; Carbaryl; Carbofuran; Chromatography, High Pressure Liquid; Human; Insecticides, Carbamate; Phenols; Propoxur |
| Is Qa | No |