Characterization of monoclonal antibodies against prostaglandin E2: fine specificity and neutralization of biological effects
David, F; Somme, G; Provostwisner, A; Roth, C; Astoin, M; Dray, F; Theze, J; ,
| HERO ID | 9216824 |
|---|---|
| In Press | No |
| Year | 1985 |
| Title | Characterization of monoclonal antibodies against prostaglandin E2: fine specificity and neutralization of biological effects |
| Authors | David, F; Somme, G; Provostwisner, A; Roth, C; Astoin, M; Dray, F; Theze, J; , |
| Journal | Molecular Immunology |
| Volume | 22 |
| Issue | 3 |
| Page Numbers | 339-346 |
| Abstract | The specificity and heterogeneity of the immune response of BALB/c mice immunized with prostaglandin E2 (PGE2) coupled to thyroglobulin was studied. All the animals (n = 50) responded to PGB2, a transformation product of PGE2. However, following repeated injections most of the animals (n = 30) were also able to respond to PGE2. Cellular hybridizations were performed and five anti-PGE2 monoclonal antibodies were isolated and analysed. They are mainly directed against the ring and the omega-chain of PGE2 but their specificity toward the alpha-chain is more limited. The association constants are greater than to 1 X 10(9) M-1. The monoclonal antibody 8E.57.71 (Ka = 1.3 X 10(10) M-1) is particularly convenient for sensitive radioimmunoassays (detection limit 25pg/ml, when iodinated tracer is used). Anti-PGE2 monoclonal antibodies were found to neutralize the specific binding of [3H]PGE2 to rat brain hypothalamic receptors and to inhibit the PGE2 induction of rat fundus muscular contraction. |
| Doi | 10.1016/0161-5890(85)90170-1 |
| Pmid | 3858663 |
| Wosid | WOS:A1985ADZ4700020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: ISSN: |
| Is Public | Yes |
| Language Text | English |