Anionic selectivity sequence of the Cl(-)-H+ symporter in the synaptosomal preparation from rat brain cortex
Torres, ML; Ortega, F; Cuaranta, I; González, J; Sanchez-Armass, S
HERO ID
900745
Reference Type
Journal Article
Year
2008
Language
English
PMID
| HERO ID | 900745 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Anionic selectivity sequence of the Cl(-)-H+ symporter in the synaptosomal preparation from rat brain cortex |
| Authors | Torres, ML; Ortega, F; Cuaranta, I; González, J; Sanchez-Armass, S |
| Journal | Neurochemical Research |
| Volume | 33 |
| Issue | 8 |
| Page Numbers | 1574-1581 |
| Abstract | The Na(+)/H(+) exchanger has been the only unequivocally demonstrated H(+)-transport mechanism in the synaptosomal preparation. We had previously suggested that a Cl(-)-H(+) symporter (in its acidifying mode) is involved in cytosolic pH regulation in the synaptosomal preparation. Supporting this suggestion, we now show that: (1) when synaptosomes are transferred from PSS to either gluconate or sulfate solutions, the Fura-2 ratio remains stable instead of increasing as it does in 50 mM K solution. This indicates that these anions do not promote a plasma membrane depolarization. (2) Based in the recovery rate from the cytosolic alkalinization, the anionic selectivity of the Cl(-)-H(+) symporter is NO(3)(-) > Br(-) > Cl(-) > I(-) = isethionate = sulfate = methanesulfonate = gluconate. (3) PCMB 10 muM inhibits the gluconate-dependent alkalinization by 30 +/- 6%. (4) Neither Niflumic acid, 9AC, Bumetanide nor CCCP inhibits the recovery from the cytosolic alkalinization. |
| Doi | 10.1007/s11064-008-9685-x |
| Pmid | 18404376 |
| Wosid | WOS:000257237900020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |