Myrrh mediates haem oxygenase-1 expression to suppress the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages
Cheng, YW; Cheah, KP; Lin, CW; Li, JS; Yu, WY; Chang, ML; Yeh, GC; Chen, SH; Choy, CS; Hu, CM
| HERO ID | 871738 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Myrrh mediates haem oxygenase-1 expression to suppress the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages |
| Authors | Cheng, YW; Cheah, KP; Lin, CW; Li, JS; Yu, WY; Chang, ML; Yeh, GC; Chen, SH; Choy, CS; Hu, CM |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 63 |
| Issue | 9 |
| Page Numbers | 1211-1218 |
| Abstract | To elucidate a novel anti-inflammatory mechanism of myrrh against lipopolysaccharide (LPS)-induced inflammation.<br /><br /> RAW264.7 macrophages were cultured in DMEM and then cells were treated with LPS or LPS plus a myrrh methanol extract (MME) for 24h. The culture medium was collected for determination of nitric oxide (NO), prostaglandin (PG)E(2) , interleukin (IL)-1β, and tumour necrosis factor (TNF)-α, and cells were harvested by lysis buffer for Western blot analysis.<br /><br /> Our data showed that treatment with the MME (1∼100µg/ml) did not cause cytotoxicity or activate haem oxygenase-1 (HO-1) protein synthesis in RAW264.7 macrophages. Furthermore, the MME inhibited LPS-stimulated NO, PGE(2) , IL-1β and TNF-α release and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. Zn(II) protoporphyrin IX, a specific inhibitor of HO-1, blocked the inhibition of iNOS and COX-2 expression by the MME.<br /><br /> These results suggest that among mechanisms of the anti-inflammatory response, the MME inhibited the production of NO, PGE(2) , IL-1β and TNF-α by downregulating iNOS and COX-2 gene expression in macrophages and worked through the action of HO-1. |
| Doi | 10.1111/j.2042-7158.2011.01329.x |
| Pmid | 21827494 |
| Wosid | WOS:000293699100011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000293699100011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | cyclooxygenase-2; haem oxygenase-1; inducible nitric oxide synthase; lipopolysaccharide; myrrh |
| Is Qa | No |