Accurate LC-MS analyses for microcystins using per-15N-labeled microcystins
Sano, T; Takagi, H; Nagano, K; Nishikawa, M; Kaya, K
HERO ID
867000
Reference Type
Journal Article
Year
2011
Language
English
PMID
| HERO ID | 867000 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Accurate LC-MS analyses for microcystins using per-15N-labeled microcystins |
| Authors | Sano, T; Takagi, H; Nagano, K; Nishikawa, M; Kaya, K |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 399 |
| Issue | 7 |
| Page Numbers | 2511-2516 |
| Abstract | Per-(15)N-labeled microcystins were prepared for use as surrogates for accurate liquid chromatography-mass spectrometry analysis. Two strains of Microcystis aeruginosa were cultured in (15)NO(3)-containing TS-15 medium. To change from the incorporation of (14)N to (15)N into all cell components, cells of Microcystis aeruginosa were precultured in Na(15)NO(3)-containing medium for more than 6 months. After mass cultivation of the strains, cells of each strain were harvested and lyophilized. Microcystin variants were extracted from the lyophilized cells and per-(15)N-labeled microcystin variants were purified using high-performance liquid chromatography and high-performance thin-layer chromatography. The structures of per-(15)N-labeled microcystin variants were confirmed by their mass spectrometry spectra and NMR spectra. When per-(15)N-labeled microcystins were used as surrogates for quantitative analysis of these toxins in cyanobacterial cells, excellent accuracy (98-106%) was obtained, with the m/z of M(+), [M+1](+), and [M+2](+) of both microcystins and the per-(15)N-labeled microcystins as surrogates being completely separated. In conclusion, per-(15)N-labeled microcystins are excellent surrogates for microcystin analysis using liquid chromatography-mass spectrometry. |
| Doi | 10.1007/s00216-010-4639-y |
| Pmid | 21221535 |
| Wosid | WOS:000287141700023 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951809634&doi=10.1007%2fs00216-010-4639-y&partnerID=40&md5=171fcf5156af02fb5a91608d6eec3e11 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Microcystin; Stable isotope; Surrogate; Liquid chromatography-mass spectrometry; Accurate analysis |
| Is Qa | No |