Antioxidant, pro-oxidant and cytotoxic properties of parsley
Dorman, HJD; Lantto, TA; Raasmaja, A; Hiltunen, R
| HERO ID | 836397 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Antioxidant, pro-oxidant and cytotoxic properties of parsley |
| Authors | Dorman, HJD; Lantto, TA; Raasmaja, A; Hiltunen, R |
| Volume | 2 |
| Issue | 6 |
| Page Numbers | 328-337 |
| Abstract | Parsley (Petroselinum crispum) leaves were macerated with a mixture of methanol: water: acetic acid to produce a crude extract which was then defatted with (40 degrees-60 degrees) petrol. Antioxidant activity of the extract was evaluated using a battery of in vitro assays, viz., iron(III) reduction, iron(II) chelation and free radical scavenging assays. Evaluation of the pro-oxidant activity of the extract was based upon its effects upon DNA fragmentation and protein carbonylation. Cytotoxicity and apoptotic effects of the extract were determined in non-cancerous CV1-P fibroblast and cancerous A375 melanoma cells using MTT and LDH tests and caspase 3-like activity assay. The highest concentration, 2.0 mg ml(-1), decreased the viability of both cell lines, however, the cancerous melanoma cells were slightly susceptible to the effects. The observed cytotoxicity was not due to the caspase 3 activity. In conclusion, the toxicity might be explained by the pro-oxidative activity of components within the extract against proteins and/or DNA but it is not related to caspase 3-dependent apoptosis within cells. |
| Doi | 10.1039/c1fo10027k |
| Pmid | 21779571 |
| Wosid | WOS:000291992800005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Food & function ISSN: 2042-650X |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |