Expression of PPARgamma and its ligand-dependent growth inhibition in human brain tumor cell lines

Kato, M; Nagaya, T; Fujieda, M; Saito, K; Yoshida, J; Seo, H

HERO ID

706366

Reference Type

Journal Article

Year

2002

Language

English

PMID

12079514

HERO ID 706366
In Press No
Year 2002
Title Expression of PPARgamma and its ligand-dependent growth inhibition in human brain tumor cell lines
Authors Kato, M; Nagaya, T; Fujieda, M; Saito, K; Yoshida, J; Seo, H
Journal Japanese Journal of Cancer Research
Volume 93
Issue 6
Page Numbers 660-666
Abstract Peroxisome proliferator-activated receptor gamma (PPARgamma) belongs to a superfamily of thyroid / steroid hormone receptors and regulates transcription of their target genes in a ligand-dependent manner. Recently, PPARgamma was reported to be expressed in several cell lines derived from breast, colon, stomach and lung cancers. Activation of PPARgamma by its ligand inhibits the growth of these tumor cells, suggesting that PPARgamma ligand is a potential anti-cancer agent in PPARgamma-expressing tumors. However, its expression in brain tumors has not been studied. We thus studied the expression in glioma samples with different pathological stages from 20 patients. It was demonstrated that 95% of the glioma tissue expressed PPARgamma mRNA. The results prompted us to study whether PPARgamma ligand affects the growth of cell lines derived from brain tumors. The receptor expression was studied in 9 cell lines either derived from malignant glioma or neuroblastoma. The expression was detected in a glioma cell line SK-MG-1 and in a neuroblastoma cell line NB-1. Addition of one of the PPARgamma ligands, troglitazone, induced growth inhibition in both cell lines. Further analyses revealed that this growth inhibition is caused by a PPARgamma-mediated induction of apoptosis. These results suggest that PPARgamma ligands could be a potential therapeutic agent for the treatment of the brain tumors expressing this receptor.
Pmid 12079514
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Language Text English
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