Marked enhancement in the reductive dehalogenation of hexachloroethane by a Thr319Ala mutation of cytochrome P450 1A2
Yanagita, K; Sagami, I; Daff, S; Shimizu, T
HERO ID
699691
Reference Type
Journal Article
Year
1998
Language
English
PMID
| HERO ID | 699691 |
|---|---|
| In Press | No |
| Year | 1998 |
| Title | Marked enhancement in the reductive dehalogenation of hexachloroethane by a Thr319Ala mutation of cytochrome P450 1A2 |
| Authors | Yanagita, K; Sagami, I; Daff, S; Shimizu, T |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 249 |
| Issue | 3 |
| Page Numbers | 678-682 |
| Abstract | Mutation of the conserved Thr319 residue to Ala of cytochrome P4501A2 (CYP1A2) increased the value of Vmax 9-fold for reductive dehalogenation of hexachloroethane in the reconstituted system under anaerobic conditions. The Thr319Ala mutation also increased the elimination over substitution product ratio by 5-fold. The addition of aliphatic alcohols increased by 22-fold the activity obtained with the wild type and varied the elimination over substitution product ratio. Increasing pH increased the ratio of elimination over substitution by primarily affecting the rate of elimination. |
| Doi | 10.1006/bbrc.1998.9084 |
| Pmid | 9731196 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |