Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: a comparison using control sera obtained from healthy individuals and patients with multiple medical problems
Wichainun, R; Kasitanon, N; Wangkaew, S; Hongsongkiat, S; Sukitawut, W; Louthrenoo, W
HERO ID
6781359
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 6781359 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: a comparison using control sera obtained from healthy individuals and patients with multiple medical problems |
| Authors | Wichainun, R; Kasitanon, N; Wangkaew, S; Hongsongkiat, S; Sukitawut, W; Louthrenoo, W |
| Journal | Asian Pacific Journal of Allergy & Immunology |
| Volume | 31 |
| Issue | 4 |
| Page Numbers | 292-298 |
| Abstract | <strong>BACKGROUND: </strong>Antinuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) are often tested as a screening tool in patients with suspected systemic lupus erythematosus or connective tissue diseases. ANA can be seen in healthy controls (HC) and patients with multiple medical problems (MMP).<br /><br /><strong>OBJECTIVE: </strong>To determine the sensitivity and specificity of ANA and anti-dsDNA in SLE patients, using sera from HC and MMP patients.<br /><br /><strong>METHODS: </strong>Serum samples from HC, MMP and SLE patients, 100 in each group, were analyzed for the presence of ANA and anti-dsDNA, by indirect immunofluorescent assay, using a HEp-2 cell and Crithidia luciliae as substrates, respectively.<br /><br /><strong>RESULTS: </strong>The prevalence of ANA at a titer of ≥1:80 and ≥ 1:160 was 8% and 4%, respectively, in HC; and it was 12% and 6% respectively, in MMP patients. The prevalence of anti-dsDNA was 0% in HC and 3% in MMP patients. When using HC sera for the diagnosis of SLE, the sensitivity of ANA at a titer of ≥ 1:80 and ≥ 1:160 was 98% and 90%, respectively, with specificity of 92% and 96%, respectively. The specificity decreased to 88% and 94%, respectively, when using sera from MMP patients. The specificity of anti-dsDNA was 100% and 97%, when using sera from HC and MMP patients, respectively.<br /><br /><strong>CONCLUSION: </strong>ANA and anti-dsDNA gave high sensitivity and high specificity in patients with SLE, even when using MMP patient's sera as controls. Physicians should take care in interpreting ANA and anti-dsDNA results in MMP patients who do not have signs or symptoms of SLE or connective tissue diseases. |
| Doi | 10.12932/AP0272.31.4.2013 |
| Pmid | 24383972 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |