Results of the L5178Y mouse lymphoma assay and the Balb/3t3 cell in vitro transformation assay for eight phthalate esters

Barber, ED; Cifone, M; Rundell, J; Przygoda, R; Astill, BD; Moran, E; Mulholland, A; Robinson, E; Schneider, B

HERO ID

673420

Reference Type

Journal Article

Year

2000

Language

English

PMID

10641018

HERO ID 673420
In Press No
Year 2000
Title Results of the L5178Y mouse lymphoma assay and the Balb/3t3 cell in vitro transformation assay for eight phthalate esters
Authors Barber, ED; Cifone, M; Rundell, J; Przygoda, R; Astill, BD; Moran, E; Mulholland, A; Robinson, E; Schneider, B
Journal Journal of Applied Toxicology
Volume 20
Issue 1
Page Numbers 69-80
Abstract Eight phthalate esters, with alcohol chain lengths of 1-11 carbon atoms and with various degrees of branching, were tested in vitro in the L5178Y mouse lymphoma mammalian cell mutation assay and in the Balb/3T3 cell transformation assay. The tests were performed as part of a voluntary testing agreement between the Chemical Manufacturers Association's Phthalate Esters Panel and the United States Environmental Protection Agency (US EPA). The esters tested were: dimethyl phthalate (DMP), di-n-butyl phthalate (DBP), butyl benzyl phthalate (BBP), di- inverted question markn-hexyl, n-octyl, n-decyl inverted question mark phthalate (610P), di-isononyl phthalate (DINP), di- inverted question markheptyl, nonyl, undecyl inverted question mark phthalate (711P), di-isodecyl phthalate (DIDP) and di-undecyl phthalate (DUP). Both DMP and DBP were found to produce significant increases in the mutant frequency in the mouse lymphoma assay in the presence but not in the absence of an Aroclor-induced rat liver activation system (S-9). Ester 610P gave equivocal results in the mouse lymphoma assay in the presence and absence of rat liver S-9. There was no indication of mutagenic potential for any of the other test materials in the mouse lymphoma assay, and none of the test materials increased transformation frequency in the Balb/3T3 cell transformation assay. Aldehyde metabolites of the de-esterified alcohols are postulated to play a role in the positive results for DMP and DBP.
Doi 10.1002/(SICI)1099-1263(200001/02)20:1<69::AID-JAT630>3.0.CO;2-2
Pmid 10641018
Wosid WOS:000085357800008
Url https://search.proquest.com/docview/17500626?accountid=171501
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
Keyword phthalate esters; mouse lymphoma assay; transformation assay
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