The in vivo-in vitro replicative DNA synthesis (RDS) test with hepatocytes prepared from male B6C3F1 mice as an early prediction assay for putative nongenotoxic (Ames-negative) mouse hepatocarcinogens
Miyagawa, M; Takasawa, H; Sugiyama, A; Inoue, Y; Murata, T; Uno, Y; Yoshikawa, K
HERO ID
661834
Reference Type
Journal Article
Year
1995
Language
English
PMID
| HERO ID | 661834 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | The in vivo-in vitro replicative DNA synthesis (RDS) test with hepatocytes prepared from male B6C3F1 mice as an early prediction assay for putative nongenotoxic (Ames-negative) mouse hepatocarcinogens |
| Authors | Miyagawa, M; Takasawa, H; Sugiyama, A; Inoue, Y; Murata, T; Uno, Y; Yoshikawa, K |
| Journal | Mutation Research: Genetic Toxicology |
| Volume | 343 |
| Issue | 2-3 |
| Page Numbers | 157-183 |
| Abstract | To assess the efficacy of the in vivo-in vitro hepatocyte replicative DNA synthesis (RDS) test as a short-term assay, 41 putative nongenotoxic (Ames-negative) mouse hepatocarcinogens, as well as 31 noncarcinogens, were examined using male 8-week-old B6C3F1 mice and an in vitro [methyl-3]thymidine-incorporation technique. Animals were exposed to the maximum tolerated dose (MTD) and 1/2 MTD of each chemical by gavage and after 24, 39 or 48 h, hepatocytes were prepared with a collagenase-perfusion technique. Assessment of the distribution of spontaneous RDS in a total of 337 control mice gave an average incidence of 0.15 +/- 0.08% within the range of 0 to 0.39% (mean +/- 3 x SE) with a 99.7% probability. Values of 0.4% or more for RDS incidences induced by test samples were therefore judged as indicating a positive response in our RDS test. Under the experimental conditions applied, 32 of 41 putative nongenotoxic hepatocarcinogens gave clear positive responses (positive sensitivity: 78%), and of 31 noncarcinogens 25 samples gave negative responses (negative specificity: 81%), thus giving an overall concordance for the RDS test with long-term findings of 79%. |
| Doi | 10.1016/0165-1218(95)90082-9 |
| Pmid | 7791810 |
| Wosid | WOS:A1995RD71200009 |
| Url | http://www.sciencedirect.com/science/article/pii/0165121895900829 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Authoring Organization: HASKELL LAB |
| Is Public | Yes |
| Language Text | English |
| Keyword | Replicative DNA synthesis; Cell proliferation; RDS test; Mouse liver; Nongenotoxic mouse hepatocarcinogens; Noncarcinogens |
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