Mouse specific-locus test for the induction of heritable gene mutations by dibromochloropropane (DBCP)
Russell, LB; Hunsicker, PR; Cacheiro, NLA
HERO ID
63541
Reference Type
Journal Article
Year
1986
Language
English
PMID
| HERO ID | 63541 |
|---|---|
| In Press | No |
| Year | 1986 |
| Title | Mouse specific-locus test for the induction of heritable gene mutations by dibromochloropropane (DBCP) |
| Authors | Russell, LB; Hunsicker, PR; Cacheiro, NLA |
| Journal | DNA Repair |
| Volume | 170 |
| Issue | 3 |
| Page Numbers | 161-166 |
| Abstract | The nematocide DBCP (1,2-dibromo-3-chloropropane) produced negative results in a specific-locus test for gene-mutation induction in the germline of male (101 X C3H)F1 mice, most of which were treated with 5 daily intraperitoneal injections of 80 mg/kg (total exposure, 400 mg/kg); a few received lower exposures. For treated spermatogonial stem cells, the finding of 2 mutations among 39519 offspring--a rate almost identical to the control rate--rules out (at the 5% significance level) an induced mutation frequency greater than 2.0 times the historical control rate. From treated poststem-cell stages, no mutants were found among 6240 offspring, ruling out (at the 5% significance level) a multiple of 8.0 times the control for these cell types. A multiple rearrangement (7 chromosomes involved in 3 translocations) found in one of the mutants probably arose as a postmeiotic event not associated with the DBCP treatment. The fertility of DBCP-treated males was not disturbed, in keeping with the absence of germ-cell toxicity and dominant lethals found by other investigators in these mice, and in contrast to results in certain other species. While the treated (101 X C3H)F1 mice are Ah-responsive, other findings make it questionable whether biotransformation of DBCP to reactive intermediates is accomplished via the Ah-receptor system. |
| Pmid | 3713725 |
| Is Certified Translation | No |
| Dupe Override | 63541 |
| Comments | Mutat. Res. 170: 161-166. |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |