Interactive effect of nitrogen source and high CO2 concentration on the growth of the dinoflagellate Alexandrium tamarense and its toxicity to zebrafish (Danio rerio) embryos

Guan, W; Si, R; Li, X; Cai, J; Chen, S

HERO ID

6332843

Reference Type

Journal Article

Year

2018

Language

English

PMID

30041358

HERO ID 6332843
In Press No
Year 2018
Title Interactive effect of nitrogen source and high CO2 concentration on the growth of the dinoflagellate Alexandrium tamarense and its toxicity to zebrafish (Danio rerio) embryos
Authors Guan, W; Si, R; Li, X; Cai, J; Chen, S
Journal Marine Pollution Bulletin
Volume 133
Page Numbers 626-635
Abstract The effects and interactive effects of different nitrogen (N) sources (ammonium, nitrate, and urea) and carbon dioxide (CO2) concentrations were investigated on Alexandrium tamarense, a harmful marine dinoflagellate, by measuring its growth (μ), extracellular carbonic anhydrase (CA), and its toxicity to zebrafish (Danio rerio) embryo. The μ and CA were influenced more strongly by CO2 concentrations rather than by N sources; significant effects of CO2 on μ and CA were observed under low CO2 concentration (LC) conditions compared to high CO2 concentration (HC) conditions. The ammonium and nitrate media under LC conditions had the maximum μ and CA, which was inhibited under HC conditions. The embryotoxic effects were influenced more strongly by the N sources than by CO2 concentrations, thus excluding the lower deformation in urea under HC conditions. Moreover, the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST), and catalase (CAT) were detected in normal (untreated) zebrafish embryos, and among them, the level of SOD was the highest. In summary, this study provides a clear insight for understanding the effects and interactive effects of N sources and CO2 concentrations on the growth and toxicity of harmful dinoflagellates.
Doi 10.1016/j.marpolbul.2018.06.024
Pmid 30041358
Wosid WOS:000441853600068
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
Keyword Nitrogen (N) source; CO2 concentrations; Alexandrium tamarense; Extracellular carbonic anhydrase (CA); Growth rate; Toxicity