Active transport of glutathione S-conjugate in human colon adenocarcinoma cells
Zhang, K; Wong, KP
HERO ID
629594
Reference Type
Journal Article
Year
1996
Language
English
PMID
| HERO ID | 629594 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Active transport of glutathione S-conjugate in human colon adenocarcinoma cells |
| Authors | Zhang, K; Wong, KP |
| Journal | Cancer Letters |
| Volume | 108 |
| Issue | 1 |
| Page Numbers | 143-151 |
| Abstract | The formation of the glutathione S-conjugate of monochlorobimane (GSH- bimane) in human colon adenocarcinoma cells was identified by HPLC- fluorimetry and its transport from the cells was found to be temperature-sensitive, saturable and ATP-dependent. The apparent K(m) and Vmax values were 2.4 +/- 0.5 nmol GSH-bimane/10(6) cells and 0.5 +/- 0.1 nmol GSH-bimane/min per 10(6) cells, respectively. This active transport of GSH-bimane was inhibited by low micromolar concentrations of classical uncouplers of oxidative phosphorylation, namely carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP), carbonylcyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (DNP). The efflux of GSH-bimane was competitively inhibited by chlorambucil (CMB) and 1-chloro-2,4-dinitrobenzene (CDNB), two other substrates of GST. This study demonstrates the presence and kinetic measurements of the glutathione S-conjugate export (GS-X) pump in human colon cancer cells, an export pump whose function has been implicated in the phenomenon of multidrug resistance. |
| Doi | 10.1016/S0304-3835(96)04457-6 |
| Pmid | 8950221 |
| Wosid | WOS:A1996VU65500020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030581607&doi=10.1016%2fS0304-3835%2896%2904457-6&partnerID=40&md5=c2e8d1a5df67bf279882726c1d67f7c2 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Glutathione S-conjugate pump; Human colon cancer cells; Monochlorobimane; Chlorambucil; Glutathione S-transferase |
| Is Qa | No |