Metabolite profiling of 14C-RDX in miniature pigs
Reddy, G
HERO ID
627916
Reference Type
Journal Article
Subtype
Abstract
Year
2006
Language
eng
| HERO ID | 627916 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2006 |
| Title | Metabolite profiling of 14C-RDX in miniature pigs |
| Authors | Reddy, G |
| Journal | Toxicologist |
| Volume | 90 |
| Issue | Suppl 1 |
| Page Numbers | 123 |
| Abstract | Cyclotrimethylenetrinitramine (RDX) has been used extensively as an explosive. Prior to this study no data were available on the metabolism of RDX in animals. Metabolism of 14C-RDX was studied in male and female miniature pigs after a one-time gavage with 41 to 44 mg/kg, (0.8 to 0.9 mCi/animal) in an aqueous suspension of 0.1% carboxymethyl cellulose. Metabolic profiles and identification of 14C-RDX-derived radioactivity in plasma, liver and urine were performed utilizing HPLC radio-scanning and LC/MS/MS analysis. Analytical standards were available for all proposed metabolites. Two HPLC columns with differing elution profiles were used for separation, quantification and tentative identification. Identifications were confirmed using LC/MS/MS. Two metabolites were isolated and identified as 4-nitro-2, 4-diazabutanal and a novel metabolite, 4-nitro- 2-4 diaza-butanamide. Analysis also revealed trace levels of 1-nitroso-3,5-dinitro-1,3,5-triazacyclohexane (MNX), 1,3-dintroso-5-nitro-1,3,5-triazacyclohexane (DNX) and 1,3,5-trinitroso- 1,3,5-triazacyclohexane (TNX) in plasma and showed trace levels of MNX and DNX in urine. No metabolites were detected in the liver samples. Thus RDX was metabolized primarily by a method that accomplished both denitration and oxidative cleavage of the ring structure of this compound to form butanal and butanamide metabolites. |
| Is Certified Translation | No |
| Dupe Override | 627916 |
| Conference Name | Society of Toxicology Annual Meeting |
| Is Public | Yes |
| Language Text | eng |
| Is Qa | No |