Mechanisms of arsenic-induced cell transformation
Barrett, JC; Lamb, PW; Wang, TC; Lee, TC
HERO ID
627590
Reference Type
Journal Article
Subtype
Review
Year
1989
Language
English
PMID
| HERO ID | 627590 |
|---|---|
| Material Type | Review |
| In Press | No |
| Year | 1989 |
| Title | Mechanisms of arsenic-induced cell transformation |
| Authors | Barrett, JC; Lamb, PW; Wang, TC; Lee, TC |
| Journal | Biological Trace Element Research |
| Volume | 21 |
| Issue | 1 |
| Page Numbers | 421-429 |
| Abstract | Arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. Studies with cells in culture have provided some possible mechanisms by which arsenic and arsenical compounds may exert a carcinogenic activity. Sodium arsenite and sodium arsenate were observed to induce morphological transformation of Syrian hamster embryo cells in a dose-dependent manner. The trivalent sodium arsenite was greater than tenfold more potent than the pentavalent sodium arsenate. The compounds also exhibited toxicity; however, transformation was observed at nontoxic as well as toxic doses. At low doses, enhanced colony forming efficiency of the cells was observed. To understand the mechanism of arsenic-induced transformation, the genetic effects of the two arsenicals were examined over the same doses that induced transformation. No arsenic-induced gene mutations were detected at two genetic loci. However, cell transformation and cytogenetic effects, including endoreduplication, chromosome aberrations, and sister chromatid exchanges, were induced by the arsenicals with similar dose responses. These results support a possible role for chromosomal changes in arsenic-induced transformation. The two arsenic salts also induced another form of mutation-gene amplification. Both sodium arsenite and sodium arsenate induced a high frequency of methotrexate-resistant 3T6 cells, which were shown to have amplified copies of the dihydrofolate reductase gene.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Doi | 10.1007/BF02917284 |
| Pmid | 2484623 |
| Wosid | WOS:A1989AV54500055 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | |WOS:A1989AV54500055 |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>General Biology-Symposia</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Microscopy Techniques-Cytology and Cytochemistry</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Cytology and Cytochemistry-Animal</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Genetics and Cytogenetics-Animal</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biochemical Studies-Minerals</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Anatomy and Histology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Toxicology-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Neoplasms and Neoplastic Agents-Carcinogens and Carcinogenesis</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Cricetidae</kw> |
| Relationship(s) |
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