Caenorhabditis elegans gcs-1 confers resistance to arsenic-induced oxidative stress
Liao, V; Yu, CW
HERO ID
627325
Reference Type
Journal Article
Year
2005
Language
English
PMID
| HERO ID | 627325 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Caenorhabditis elegans gcs-1 confers resistance to arsenic-induced oxidative stress |
| Authors | Liao, V; Yu, CW |
| Journal | BioMetals |
| Volume | 18 |
| Issue | 5 |
| Page Numbers | 519-528 |
| Abstract | Gamma-glutamylcysteine synthetase (gamma-GCS) catalyzes the first, rate-limiting step in the biosynthesis of glutathione (GSH). To evaluate the protective role of cellular GSH against arsenic-induced oxidative stress in Caenorhabditis elegans (C. elegans), we examined the effect of the C. elegans ortholog of GCS(h), gcs-1, in response to inorganic arsenic exposure. We have evaluated the responses of wild-type and gcs-1 mutant nematodes to both inorganic arsenite (As(III)) and arsenate (As(V)) ions and found that gcs-1 mutant nematodes are more sensitive to arsenic toxicity than that of wild-type animals. The amount of metal ion required to kill half of the population of worms falls in the order of wild-type/As(V)>gcs-1/As(V)> wild-type/As(III)>gcs-1/As(III). gcs-1 mutant nematodes also showed an earlier response to the exposure of As(III) and As(V) than that of wild-type animals. Pretreatment with GSH significantly raised the survival rate of gcs-1 mutant worms compared to As(III)- or As(V)-treated worms alone. These results indicate that GCS-1 is essential for the synthesis of intracellular GSH in C. elegans and consequently that the intracellular GSH status plays a critical role in protection of C. elegans from arsenic-induced oxidative stress. |
| Doi | 10.1007/s10534-005-2996-3 |
| Pmid | 16333752 |
| Wosid | WOS:000233823500007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | |WOS:000233823500007 |
| Is Public | Yes |
| Language Text | English |
| Keyword | gamma-glutamylcysteine synthetase; glutathione; Caenorhabditis elegans; arsenic |
| Is Qa | No |