Induction of DNA damage by free radicals generated either by organic or inorganic arsenic (AsIII, MMAIII, and DMAIII) in cultures of B and T lymphocytes
Gómez, SE; del Razo, LM; Muñoz Sanchez, JL
HERO ID
627243
Reference Type
Journal Article
Year
2005
Language
English
PMID
| HERO ID | 627243 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Induction of DNA damage by free radicals generated either by organic or inorganic arsenic (AsIII, MMAIII, and DMAIII) in cultures of B and T lymphocytes |
| Authors | Gómez, SE; del Razo, LM; Muñoz Sanchez, JL |
| Journal | Biological Trace Element Research |
| Volume | 108 |
| Issue | 1-3 |
| Page Numbers | 115-126 |
| Abstract | The aim of this work is based in the premise that inorganic arsenic (AsIII) and trivalentmethylated metabolites monomethylarsonous (MMAIII) and dimethylarsinous (DMAIII) participate in DNA damage through the generation of reactive oxygen species (ROS). We have utilized two lymphoblastic lines, Raji (B cells) and Jurkat (T cells), which were treated with the trivalent arsenic species (dose: 0-100 microM) and analyzed by two assays (comet assay and flow cytometry) in the determination of DNA damage and ROS effects in vivo. The results showed that the damage to the DNA and the generation of ROS are different in both cellular lines with respect to the dose of organic arsenic, and the order of damage is MMAIII>DMAIII>AsIII. This fact suggests that the DMAIII is not always the more cytotoxic intermediary xenobiotic, as has already been reported in another study. |
| Doi | 10.1385/BTER:108:1-3:115 |
| Pmid | 16327065 |
| Wosid | WOS:000234115400011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | |WOS:000234115400011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | DNA damage; trivalent arsenic; reactive oxygen species (ROS); comet assay; dihydrorhodamine 123 (DHR123); flow cytometry |
| Is Qa | No |