Enzyme levels in rat testis damaged experimentally with dibutyl phthalate
Tanino, M; Ikemoto, I; Tanaka, A
| HERO ID | 61580 |
|---|---|
| In Press | No |
| Year | 1987 |
| Title | Enzyme levels in rat testis damaged experimentally with dibutyl phthalate |
| Authors | Tanino, M; Ikemoto, I; Tanaka, A |
| Journal | Jikeikai Medical Journal |
| Volume | 34 |
| Issue | 2 |
| Page Numbers | 245-252 |
| Abstract | In order to study the biochemical changes involved in the testicular damage induced by phthalate ester plasticizers, dibutyl phthalate (DBP), a representative plasticizer, was administered orally to mature male rats for one week. The rats were sequently killed at 1, 7, 14, and 21 days after the final administration of DBP. One testis was examined histologically and the other was subjected to assay for the activities of the testicular enzymes lactate dehydrogenase-X (LDH-X) and ornithine decarboxylase (ODC). On day I, the testes were clearly atrophied. Histologically, the germ cells had almost disappeared in the seminiferous tubules. On day 21, the testicular damage had mostly recovered. Enzyme assay showed that LDH-X activity was depressed severely on day I, but recovered with time. Therefore, its activity showed good correlation with the histological changes in the testis. ODC activity was depressed on day I but elevated on day 14. This indicated that the recovery of ODC activity preceded the histological recovery. Because the pathological findings obtained in these damaged testes resembled to those of human male infertility, the changes in the activities of the two testicular enzymes after DBP treatment may provide a clue toward the understanding of the pathophysiological phenomena involved in clinical infertile testis. |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Jikeikai Med. J. 34: 245-252. |
| Is Public | Yes |
| Language Text | English |
| Keyword | testicular damage; male infentility; phthalic acid; ornithine decarboxylase; lactate dehydrogenase isoenzymes |
| Is Peer Review | Yes |