Supercritical-fluid extraction of bilobalide and ginkgolides from Ginkgo biloba leaves by use of a mixture of carbon dioxide, methanol, and water

Choi, YH; Kim, J; Yoo, KP

HERO ID

5778961

Reference Type

Journal Article

Year

2002

Language

English

HERO ID 5778961
In Press No
Year 2002
Title Supercritical-fluid extraction of bilobalide and ginkgolides from Ginkgo biloba leaves by use of a mixture of carbon dioxide, methanol, and water
Authors Choi, YH; Kim, J; Yoo, KP
Journal Chromatographia
Volume 56
Issue 11-12
Page Numbers 753-757
Abstract Supercritical-fluid extraction (SFE) with pure carbon dioxide and with carbon dioxide modified by addition of methanol, ethanol, or water have been used to extract bilobalide and ginkgolides A, B, and C from Ginkgo biloba leaves. Each extract was analyzed by HPLC-ESIMS. Although addition of methanol or ethanol led to enhonced SFE yields of the compounds compared with use of pure carbon dioxide, the amounts extracted were only 10% those obtained by extraction with an organic solvent such as methanol or acetone-water 1:1. Addition of small amounts (4%, v/v) of water as modifier of the carbon dioxide, however, led to a dramatic improvement in the extraction yield of the compounds - to amounts which were 80-150% of the yields obtained by extraction with organic solvent. In addition, levels of ginkgolic acids, the toxic constituents of Ginkgo biloba leaves, extracted by SFE with carbon dioxide-methanol-water, 80:16:4, were substantially lower after continuous pre-extraction with n-hexane and with pure carbon dioxide.
Doi 10.1007/BF02492479
Wosid WOS:000179861600015
Is Certified Translation No
Dupe Override No
Comments Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036907199&doi=10.1007%2fBF02492479&partnerID=40&md5=0c222c56681bb91f5956fe6ebff4eab6
Is Public Yes
Language Text English
Keyword supercritical-fluid extraction; selective extraction; Ginkgo biloba; ginkgolides A, B, C; ginkgolic acid