Determination of ergosterol in canola (Brassica napus L.) by liquid chromatography
Abramson, D; Smith, DM
| HERO ID | 5778933 |
|---|---|
| In Press | No |
| Year | 2002 |
| Title | Determination of ergosterol in canola (Brassica napus L.) by liquid chromatography |
| Authors | Abramson, D; Smith, DM |
| Journal | Journal of Stored Products Research |
| Volume | 39 |
| Issue | 2 |
| Page Numbers | 185-191 |
| Abstract | A sensitive and precise method is described to assay the oilseed canola (Brassica napus L.) for ergosterol, a fungal metabolite indicating spoilage. The ground seed is refluxed in methanol, and the methanol extract is saponified with potassium hydroxide. After addition of water, the mixture is partitioned into n-hexane. The n-hexane extract is dried, reconstituted, and applied to a silica solid-phase extraction cartridge, which is then washed with carbon tetrachloride, and eluted with acetone. The acetone eluate is acetylated, and the ergosterol determined as the acetate by liquid chromatography using a reverse-phase column and absorbency detection at 282 nm. Acetylation is necessary since an unidentified constituent of the matrix co-chromatographs with free ergosterol, and the ergosterol peak cannot otherwise be resolved. Using a 2-h acetylation at 60°C, recoveries of 10 and 25 ppm added ergosterol were 96.3% and 94.0%, respectively (n = 4, relative SD=3.8% in each case). Results using a convenient overnight acetylation at 37°C were similar and not significantly different. The limit of detection was approximately 0.3 ppm. Crown Copyright © 2002 Published by Elsevier Science Ltd. All rights reserved. |
| Doi | 10.1016/S0022-474X(01)00052-2 |
| Wosid | WOS:000179979700006 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037032761&doi=10.1016%2fS0022-474X%2801%2900052-2&partnerID=40&md5=98c22da7b7132ae370c7365df1c51017 |
| Is Public | Yes |
| Language Text | English |
| Keyword | rapeseed; canola; ergosterol; chromatography; spoilage |