Batch test for assessing the mineralization of C-14-radiolabeled compounds under realistic anaerobic conditions
Nuck, BA; Federle, TW
HERO ID
5094384
Reference Type
Journal Article
Year
1996
Language
English
| HERO ID | 5094384 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Batch test for assessing the mineralization of C-14-radiolabeled compounds under realistic anaerobic conditions |
| Authors | Nuck, BA; Federle, TW |
| Journal | Environmental Science & Technology |
| Volume | 30 |
| Issue | 12 |
| Page Numbers | 3597-3603 |
| Abstract | A test system is described for assessing the mineralization of 14C-radiolabeled compounds to carbon dioxide (CO2) and methane (CH4) under anaerobic conditions. Environmentally realistic concentrations of test chemicals were incubated with anaerobic digester sludge in sealed vessels at 35 °C. The headspace of these vessels was continuously purged with nitrogen, which was passed through a series of potassium hydroxide (KOH) scrubbers to trap 14CO2. This effluent gas was mixed with oxygen and passed through a combustion tube containing cupric oxide at 800 °C to convert 14CH4 to 14CO2, which was subsequently trapped in a second series of KOH traps. Trapping efficiency was 97.1 ± 5% for 14CO2 and 108 ± 13% for 14CH4. The system was used to evaluate the mineralization of several organic chemicals including glucose, palmitic acid, hexadecanol, and two surfactants, alkyl ethoxylate sulfate (AES) and alkyl sulfate (AS), in anaerobic digester sludge. Total yields of 14CH4 and 14CO2 from these compounds exceeded 80%, and the total mass balances generally exceeded 95%. Mineralization of hexadecanol was described by a simple first-order production equation, while that of the other compounds was best described by a three-half-order model. This system provided excellent recovery of radioactive gases and consequently excellent mass balances. Given the reproduction of in situ condition and the realistic ratio of test material to biomass, the test system also generated relevant kinetic data. It, therefore, has application for demonstrating the significance of biodegradation as a removal mechanism during anaerobic digestion as well as determining the completeness of anaerobic biodegradation. |
| Doi | 10.1021/es960302u |
| Wosid | WOS:A1996VV20500050 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |