INFLUENCE OF DIETARY CALCIUM AND VITAMIN-D ON COLON EPITHELIAL-CELL PROLIFERATION AND 1,2-DIMETHYLHYDRAZINE-INDUCED COLON CARCINOGENESIS IN RATS FED HIGH-FAT DIETS

Beaty, MM; Lee, EY; Glauert, HP

HERO ID

4947317

Reference Type

Journal Article

Year

1993

HERO ID 4947317
In Press No
Year 1993
Title INFLUENCE OF DIETARY CALCIUM AND VITAMIN-D ON COLON EPITHELIAL-CELL PROLIFERATION AND 1,2-DIMETHYLHYDRAZINE-INDUCED COLON CARCINOGENESIS IN RATS FED HIGH-FAT DIETS
Authors Beaty, MM; Lee, EY; Glauert, HP
Journal Journal of Nutrition
Volume 123
Issue 1
Page Numbers 144-152
Abstract We investigated whether increased levels of dietary calcium and vitamin D could inhibit colon carcinogenesis in rats injected with a single dose of 1,2-dimethylhydrazine. Rats were given a single subcutaneous injection (200 mg/kg body wt) 2 wk before they were fed purified diets containing 20% fat for 32 wk. Diets contained one of three levels of calcium (5, 10 or 15 g/kg diet) as calcium gluconate and one of three levels of vitamin D (0.025, 0.05 or 0.1 mg/kg diet) as cholecalciferol in a 3 x 3 factorial design. Rats receiving the highest level of vitamin D had greater plasma concentrations of 25-hydroxy-vitamin D. Autoradiographic examination of [H-3]thymidine-treated rats demonstrated that a higher dietary level of calcium as well as higher levels of vitamin D significantly affected cellular kinetic indices. The total tumor incidence and tumor incidence in the distal colon was 45% lower in rats fed the highest level of both calcium and vitamin D compared with the other eight groups, although this decrease was not statistically significant (P = 0.12). The possible importance of these observations is discussed.
Wosid WOS:A1993KG33100017
Is Certified Translation No
Dupe Override 4947317
Is Public Yes
Keyword COLON CANCER; 1,2-DIMETHYLHYDRAZINE; CALCIUM; VITAMIN-D; RATS