AUTOMATED MONITORING OF BIOTECHNOLOGICAL PROCESSES USING ONLINE ULTRAFILTRATION AND COLUMN LIQUID-CHROMATOGRAPHY
Vandemerbel, NC; Lingeman, H; Brinkman, UAT; Kolhorn, A; Derijke, LC
| HERO ID | 4947288 |
|---|---|
| In Press | No |
| Year | 1993 |
| Title | AUTOMATED MONITORING OF BIOTECHNOLOGICAL PROCESSES USING ONLINE ULTRAFILTRATION AND COLUMN LIQUID-CHROMATOGRAPHY |
| Authors | Vandemerbel, NC; Lingeman, H; Brinkman, UAT; Kolhorn, A; Derijke, LC |
| Journal | Analytica Chimica Acta |
| Volume | 279 |
| Issue | 1 |
| Page Numbers | 39-50 |
| Abstract | The efficient control of fermentation processes requires reliable monitoring systems. This paper describes a fully automated monitoring system based on the on-line combination of ultrafiltration and column liquid chromatography. An evaluation of the performance of a hollow-fibre ultrafiltration module is provided and a method for the determination of sugars, alcohols and organic acids during a gluconic acid and a beer fermentation is presented. The system can be used for at least 250 h with complex fermentation broths under both aerobic and anaerobic conditions without any detrimental effect on either the fermentation or the chromatographic part. Depending on the application 3-5 analyses can be performed every hour, using only 3.0 mul of sample per analysis. Using refractometric or direct UV detection the linear dynamic range is from 0.2 to 150 g l-1 for most of the compounds, while with a simple post-column derivatization procedure sugars can be determined in the low mg l-1 range. |
| Doi | 10.1016/0003-2670(93)85064-Q |
| Wosid | WOS:A1993LK10500005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | LIQUID CHROMATOGRAPHY; ALCOHOLS; BIOTECHNOLOGY; ONLINE FERMENTATION MONITORING; ORGANIC ACIDS; SUGARS; ULTRAFILTRATION |