APPLICABILITY OF A SKIN-TEST TO THE EVALUATION OF THE ACTUAL LEVEL OF CELLULAR-IMMUNITY AND ITS LEVEL AFTER AN EXPERIMENTAL APPLICATION OF GLUCAN AND LEVAMISOL IN FATTENING BULLS
Paulik, S; Svrcek, S; Benisek, Z; Huska, M; Durove, A
| HERO ID | 4947233 |
|---|---|
| In Press | No |
| Year | 1992 |
| Title | APPLICABILITY OF A SKIN-TEST TO THE EVALUATION OF THE ACTUAL LEVEL OF CELLULAR-IMMUNITY AND ITS LEVEL AFTER AN EXPERIMENTAL APPLICATION OF GLUCAN AND LEVAMISOL IN FATTENING BULLS |
| Authors | Paulik, S; Svrcek, S; Benisek, Z; Huska, M; Durove, A |
| Journal | Veterinární Medicína |
| Volume | 37 |
| Issue | 4 |
| Page Numbers | 203-212 |
| Abstract | Applicability of a skin test induced by dinitrofluorobenzene [DNFB] to quantification of the actual level of cellular immunity [CI] in vivo and its level after an experimental immunomodulation intervention were evaluated in two breeds [40 animals in each] of fattening bulls [10-11 months old]. At the selected methodical procedure of intensity determination of the delayed type of hypersensitivity [DTH], its average value reached 4.5 + 1.5 mm in 80 animals, while in 77.5 % of bulls its level ranged from 3.6 to 9.6 mm. in 18.7 % from 2.0 to 3.5 mm and in 3.8 % remained less than 2.0 mm Evident expression of the reaction points to the possibility of application of the used methodical procedure of the skin test using DNFB to quantify the level of CI response in vivo in cattle. Percentual representation of animals according to the intensity of skin reaction [Tab. I] and concentration of total serum immunoglobulins [CS-Ig] and serum IgG [Tab. II] indicates the different cellular and humoral state of animals in investigated breeds. This is also confirmed by the recorded average values of mentioned parameters which were significantly lower [P < 0.01; or 0.05] In animals of the first breed [4.0 +/- 1.3 mm; 28.3 +/- 4.4 U ZST, 18.4 +/- 3.5 g . l-1] than in breed 2 [4.9 +/- 1.6 mm: 32.5 +/- 3.8 U ZST; 20.3 +/- 3.5 g . l-1]. The animals of each breed were divided into four experimental groups with the approximately equal actual levels of DTH [Tab. III]. Immunomodulation effects of a single administration of soluble fungal glucal [Pleurotus ostreatus] at the dose 5 mg [RFG 5 group] and 10 mg . kg-1 [RFG 10 group] and levamisol [Nilverm inj.] at the dose 5 mg . kg-1 [L 5 group] on CI response tested on day 30 after application were compared to the control. While animals of breed 1 demonstrated positive glucal effects. for both doses used on DTH reaction to DNFB, both in comparison to its level before application [P < 0.5] and to the level of control animals after its application [P < 0.05; or 0.01], [Tab. III]. similar effects of glucan were recorded in the bulls of breed 2 no. Potentiating effects of levamisol [5 mg . kg-1] on DTH to DNFB were observed in none of the breeds. |
| Wosid | WOS:A1992JK49900002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | FATTENING BULLS; SKIN TEST; CELLULAR IMMUNITY; DINITROFLUOROBENZENE; IMMUNOMODULATION; GLUCAN; LEVAMISOL; SERUM IMMUNOGLOBULINS |