Influence of culture medium and incubation time on the fatty acid compositions of some rapid-growing mycobacteria as analysed by packed and capillary column gas chromatography
Larsson, L; Valero-Guillen, P; Martin-Luengo, F; Pacheco, F
| HERO ID | 4944304 |
|---|---|
| In Press | No |
| Year | 1985 |
| Title | Influence of culture medium and incubation time on the fatty acid compositions of some rapid-growing mycobacteria as analysed by packed and capillary column gas chromatography |
| Authors | Larsson, L; Valero-Guillen, P; Martin-Luengo, F; Pacheco, F |
| Volume | 93 |
| Issue | 5 (1985) |
| Page Numbers | 353-357 |
| Abstract | Cellular fatty acids of four rapid-growing mycobacterial species (Mycobacterium chelonei, M. fortuitum, M. phlei , and M. smegmatis ) were analysed by packed and capillary column gas chromatography after one, three, four, six, eight, and twelve days of incubation on Loewenstein-Jensen and Sauton agar media. Variations in incubation time did not affect the chromatograms except in the case of twelve-day incubated M. smegmatis . Mycobacteria cultivated on Sauton agar medium contained more tuberculostearic and less oleic acid compared with Loewenstein-Jensen. For informative and reproducible analytical results, the authors recommend using a chemically defined culture medium and splitless injection on a capillary column capable of separating not only the methyl esters of the cellular fatty acids acids but also the diagnostically important secondary alcohols containing 18 and 20 carbon atoms. |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal:ACTA PATHOL., MICROBIOL. IMMUNOL. SCAND., SECT. B. ISSN: |
| Is Public | Yes |
| Keyword | Mycobacterium; fatty acid composition; gas chromatography; media (culture) |