Application of 2-factorial design on the enhanced production of calcium gluconate by a mutant strain of Aspergillus niger
Mariam, I; Nagra, SA; Haq, I; Ali, S
| HERO ID | 4942318 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Application of 2-factorial design on the enhanced production of calcium gluconate by a mutant strain of Aspergillus niger |
| Authors | Mariam, I; Nagra, SA; Haq, I; Ali, S |
| Journal | Bioresource Technology |
| Volume | 101 |
| Issue | 11 |
| Page Numbers | 4075-4080 |
| Abstract | Sixty-eight isolates of the filamentous fungus Aspergillus niger were examined for calcium gluconate production under submerged culture conditions in 500-ml Erlenmeyer flasks. The isolate Chem-15 was selected for improvement through ultraviolet (UV) light-induced mutagenesis. Among viable mutants, strain 32 exhibited the best gluconate productivity, and it was subjected to N-methyl N-nitro N-nitroso guanidine (NG) treatment. Mutant strain NG-7 gave the highest gluconate production (86.48g/L) which varied significantly (p0.05) from that of the wild type. The mutant was cultured overnight and plated on 5-fluorocytosine-PDA medium. Gluconate productivity was increased by 35% when the process parameters, incubation period (72h), initial pH (6.5), glucose as carbon source (15%), inoculum size (1.875x10(6)CFU/ml) and corn steep liquor (CSL) as nitrogen source (0.5%) were optimized using a 2-factorial Plackett-Burman design. Maximal glucose oxidase activity (28U/ml/min) was achieved at the optimal fermentation conditions with 26.5g/L DCM. The model terms were highly significant thus suggesting the potential commercial utility of the mutant (HS, df=3 approximately 0.0182). |
| Doi | 10.1016/j.biortech.2009.12.122 |
| Pmid | 20129776 |
| Wosid | WOS:000275999800040 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Glucose oxidase; Calcium; Nitrogen sources; Fermentation; Glucose; Guanidine; Carbon sources; Light effects; Mutagenesis; U.V. radiation; Colony-forming cells; Inoculum; pH effects; pH; corn; Design; Mutants; Aspergillus niger |
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