Bioavailability, biodistribution and toxicity of Biocal (TM) a new calcium source. Comparative studies in rats
Sarabia, MI; Zubillaga, M; Salgueiro, J; Lysionek, A; De Paoli, T; Hager, A; Ettlin, E; Caro, R; Weill, R; Boccio, J
| HERO ID | 4941343 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Bioavailability, biodistribution and toxicity of Biocal (TM) a new calcium source. Comparative studies in rats |
| Authors | Sarabia, MI; Zubillaga, M; Salgueiro, J; Lysionek, A; De Paoli, T; Hager, A; Ettlin, E; Caro, R; Weill, R; Boccio, J |
| Journal | Nutrition Research |
| Volume | 19 |
| Issue | 8 |
| Page Numbers | 1223-1231 |
| Abstract | The purpose of this study is to determine the bioavailability, biodistribution and toxicity of Biocal(TM), a new calcium source. Biocal(TM) is a calcium gluconate stabilized with glycine. A comparative study of this compound versus calcium gluconate was performed in Sprague-Dawley rats. Bioavailability studies were carried out by the labeling of both compounds with Ca-45. We administered a dose of 30 mg of Ca per kg of body weight p.o. to two groups of 7 male adult rats each. The urine elimination of the Ca-45, expressed as total accumulated percentage of Ca-45 activity in urine (Ae(infinity)), between the rats that received Biocal(TM) (Ae(infinity) = 2.436+/-1.337 %) and the rats that received calcium gluconate (Ae(infinity) = 1.241+/-0.473 %) were found to be statistically different (p<0.05). Biodistribution studies showed that the calcium from Biocal(TM) follows the same metabolic pathway as calcium from calcium gluconate. Values of radioactivity concentration of 97.1+/-1.3% and 98.7+/-1.6% were found in bone for Biocal(TM) and calcium gluconate, respectively. Toxicity studies of Biocal(TM) were carried out with 60 female and 60 male rats. The values of oral LD50 for female rats was 13.5 g/kg with a lower limit of 12.8 g/kg and upper limit of 14.3 g/kg. In the case of male rats the LD50 was 13.0 g/kg with a lower limit of 12.2 g/kg and upper limit of 13.9 g/kg. These values are higher with regard to the oral LD50 for calcium gluconate (10 g/kg). Our results demonstrate that calcium from Biocal(TM) has a higher bioavailability with the same metabolic behavior than calcium from calcium gluconate. The value of oral LD50 shows that the toxicity of Biocal(TM) is lower than that of the calcium gluconate. Therefore we conclude that Biocal(TM) has adequate properties to be considered as a promissory calcium compound to be used as dietary supplement or for food fortification. (C) 1999 Elsevier Science Inc. |
| Doi | 10.1016/S0271-5317(99)00083-4 |
| Wosid | WOS:000081128400011 |
| Url | http://linkinghub.elsevier.com/retrieve/pii/S0271531799000834 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | calcium; rat; bioavailability; metabolism; toxicity; solubility |