Biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-O-[1'-14C]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture
Achterberg, V; Fricke, H; Gercken, G
| HERO ID | 4929637 |
|---|---|
| In Press | No |
| Year | 1986 |
| Title | Biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-O-[1'-14C]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture |
| Authors | Achterberg, V; Fricke, H; Gercken, G |
| Journal | Chemistry and Physics of Lipids |
| Volume | 41 |
| Issue | 2 |
| Page Numbers | 137-145 |
| Abstract | Ethanolamine plasmalogen radiolabelled mainly in the O-alkenyl moiety was prepared from cell suspension cultures of the flagellate Leishmania donovani previously incubated with [1-14C]octadecanol over one growth period. The optimal concentration of [1-14C]octadecanol for labelling was shown to be 1 microM, when 60% of total lipid radioactivity appeared in the 1,2-diradyl-sn-glycero-3-phosphoethanolamine fraction, with an overall yield of approx. 35%. Analysis of this fraction revealed that 93% of the label was present in O-octa-dec-1-enyl, 3% in O-alkyl and 4% in acyl moieties. A specific radioactivity of approx. 14 mCi/mmol was determined. Raising the culture medium concentration of [1-14C]octadecanol to 2 microM yielded a product with a specific radioactivity of 25 mCi/mmol. |
| Pmid | 3536139 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |