Extractive acetone-butanol-ethanol fermentation using methylated crude palm oil as extractant in batch culture of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)
Ishizaki, A; Michiwaki, S; Crabbe, E; Kobayashi, G; Sonomoto, K; Yoshino, S
| HERO ID | 4929431 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Extractive acetone-butanol-ethanol fermentation using methylated crude palm oil as extractant in batch culture of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) |
| Authors | Ishizaki, A; Michiwaki, S; Crabbe, E; Kobayashi, G; Sonomoto, K; Yoshino, S |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 87 |
| Issue | 3 |
| Page Numbers | 352-356 |
| Abstract | The possibility of employing methylated crude palm oil (CPOE) as an extraction solvent to reduce end-product inhibition and to enhance solvent productivity in acetone-butanol-ethanol (ABE) fermentation was evaluated using oleyl alcohol as the standard butanol extractant. Fermentation was carried out at an initial glucose concentration of 90 g/l. CPOE did not inhibit the growth of the fermentative organism. Without solvent extraction, butanol production ceased after 30 h at a concentration of 15.4 g/l limiting cell growth to 3.98 g/l and glucose consumption to 62%. Applying CPOE as the extraction solvent, about 47% of the total butanol produced was extracted, glucose consumption was increased to 83% and relatively high glucose consumption rates and solvent productivities were obtained. Butanol production increased to 20.9 g/l; total ABE solvents and yield also increased from 21.2 g/l and 38% (in conventional fermentation) to 29.8 g/l and 40.4%, respectively. |
| Doi | 10.1016/S1389-1723(99)80044-9 |
| Pmid | 16232480 |
| Wosid | WOS:000081030600016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | methylated crude palm oil; extractive fermentation; Clostridium saccharoperbutylacetonicum |