Estradiol and calcium affect the growth hormone producing cells in female middle-aged rats
Milosevic, V; Trifunovic, S; Sosic-Jurjevic, B; Bujsic, N; Sekulic, M
| HERO ID | 4850280 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Estradiol and calcium affect the growth hormone producing cells in female middle-aged rats |
| Authors | Milosevic, V; Trifunovic, S; Sosic-Jurjevic, B; Bujsic, N; Sekulic, M |
| Journal | Acta Veterinaria |
| Volume | 55 |
| Issue | 2-3 |
| Page Numbers | 111-120 |
| Abstract | The effects of multiple doses of estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) on the growth and function of pituitary somatotropes (GH cells) were studied. Female middle-aged rats were receiving i.p. EDP (0.625 mg i.p./kg b.w), or Ca (11.4 mg/kg b.w) every day for two weeks. Blood samples were collected for hormone analyses and pituitaries dissected for histological and morphometric evaluation 24 h after the last injection. GH-producing cells were examined using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Both EDP- and Ca-treatment significantly decreased all morphometric parameters of GH cells (p < 0.05) in comparison with the corresponding controls. Serum concentration of growth hormone (GH) in EDP- or Ca-treated groups was lower by 65% and 13% (p < 0.05) respectively, comparing to the controls. The difference between all morphometric parameters of EDPL and Ca-treated rats was statistically significant (p<0.05) in relation to the controls. These findings suggest that multiple EDP, or Ca application affects (directly or indirectly) the control of growth and secretory activity of GH cells in middle-aged female rats. |
| Doi | 10.2298/AVB0503111M |
| Wosid | WOS:000229817500003 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | estradiol; GH cells; growth hormone; middle aged; rat females |