Nitrate interference with potassium-selective microelectrodes
Cuin, TA; Miller, AJ; Laurie, SA; Leigh, RA
| HERO ID | 4829070 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Nitrate interference with potassium-selective microelectrodes |
| Authors | Cuin, TA; Miller, AJ; Laurie, SA; Leigh, RA |
| Journal | Journal of Experimental Botany |
| Volume | 50 |
| Issue | 340 |
| Page Numbers | 1709-1712 |
| Abstract | Initial attempts to measure K+ activity (a(k)) in vacuoles of barley leaf epidermal cells using triple-barrelled K+-Selective microelectrodes gave values that were only about one-third of those expected. This was due to high (c. 200 mM) NO3- concentrations in the vacuoles interfering with the K+-sensor, The effect of NO3- was on 1,2,-dimethyl-3-nitrobenzene (DNB) used as a plasticizer in the K+-sensor. Replacing DNB with dibutyl sebacate, but not with 2-nitrophenyl octyl ether, overcame this problem and the modified sensor gave acceptable calibration curves with no interference from physiological concentrations of other ions, These electrodes were used successfully to measure a mean a(k) of 235 mM in vacuoles of epidermal cells of K+-replete barley leaves. |
| Doi | 10.1093/jexbot/50.340.1709 |
| Wosid | WOS:000083652400011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | leaf epidermis; potassium activity; potassium-selective microelectrodes; vacuoles; nitrate interference |
| Relationship(s) |
|