ATP-dependent substrate reduction at an [Fe8S9] double-cubane cluster
Jeoung, JH; Dobbek, H
| HERO ID | 4736529 |
|---|---|
| In Press | No |
| Year | 2018 |
| Title | ATP-dependent substrate reduction at an [Fe8S9] double-cubane cluster |
| Authors | Jeoung, JH; Dobbek, H |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 115 |
| Issue | 12 |
| Page Numbers | 2994-2999 |
| Abstract | Chemically demanding reductive conversions in biology, such as the reduction of dinitrogen to ammonia or the Birch-type reduction of aromatic compounds, depend on Fe/S-cluster-containing ATPases. These reductions are typically catalyzed by two-component systems, in which an Fe/S-cluster-containing ATPase energizes an electron to reduce a metal site on the acceptor protein that drives the reductive reaction. Here, we show a two-component system featuring a double-cubane [Fe8S9]-cluster [{Fe4S4(SCys)3}2(μ2-S)]. The double-cubane-cluster-containing enzyme is capable of reducing small molecules, such as acetylene (C2H2), azide (N3-), and hydrazine (N2H4). We thus present a class of metalloenzymes akin in fold, metal clusters, and reactivity to nitrogenases. |
| Doi | 10.1073/pnas.1720489115 |
| Pmid | 29507223 |
| Wosid | WOS:000427829500060 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |