Organic modifiers for the separation of organic acids and bases by liquid chromatography
Li, S; Fritz, JS
| HERO ID | 4674220 |
|---|---|
| In Press | No |
| Year | 2002 |
| Title | Organic modifiers for the separation of organic acids and bases by liquid chromatography |
| Authors | Li, S; Fritz, JS |
| Journal | Journal of Chromatography A |
| Volume | 964 |
| Issue | 1-2 |
| Page Numbers | 91-98 |
| Abstract | A straight-chain alcohol or diol additive in the mobile phase was used to modify and improve the HPLC separation of organic acids and bases. Incorporation of 2% 1-butanol, 1% 1,2-hexanediol, or 0.25% 1,2-octanediol into an aqueous mobile phase greatly improved the separation of alkane carboxylic acids on a silica C18 column, both in terms of separation time and peak shape. When 1.5% 1-hexanol, 0.09% 1-decanol or 0.01% 1-dodecanol was added to an acetonitrile-water (30:70) mobile phase, much sharper peaks and better resolution were obtained for aromatic bases separated on an underivatized polystyrene-divinylbenzene column. The mobile phase additive is believed to coat the stationary phase surface by a dynamic equilibrium. The coated surface is more hydrophilic and facilitates the efficient partitioning of analytes between the mobile and stationary phases. |
| Doi | 10.1016/S0021-9673(02)00584-8 |
| Pmid | 12198859 |
| Wosid | WOS:000177201700009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | stationary phases; LC; mobile phase composition; organic acids; organic bases |