[Effect of formaldehyde exposure on the level of cytokines in human bronchial epitheial 16HBE cells]
Yaqng, F; Yu, Y; Wang, K; Zhang, H; Wang, H; Wang, R; Yi, J
| HERO ID | 4476912 |
|---|---|
| In Press | No |
| Year | 2016 |
| Title | [Effect of formaldehyde exposure on the level of cytokines in human bronchial epitheial 16HBE cells] |
| Authors | Yaqng, F; Yu, Y; Wang, K; Zhang, H; Wang, H; Wang, R; Yi, J |
| Journal | Zhonghua Laodong Weisheng Zhiyebing Zazhi / Chinese Journal of Industrial Hygiene and Occupational Diseases |
| Volume | 34 |
| Issue | 1 |
| Page Numbers | 27-31 |
| Abstract | <strong>OBJECTIVE: </strong>To investigate the effect of formaldehyde exposure on the expression of inflammatory cytokines in human bronchial epithelial cells (16HBE cells).<br /><br /><strong>METHODS: </strong>16HBE cells were treated with formaldehyde with a concentration of 0, 0.04, 0.08, 0.16, 0.32, or 0.64 mmol/L for 24 hours, and MTT assay was applied to measure proliferative activity and calculate median lethal dose; 16HBE cells were exposed to formaldehyde with a concentration of 0, 0.04, 0.16, 0.64, or 1.20 mmol/L for 4 hours, MTT assay was applied to measure proliferative activity, and enzyme-linked immunosorbent assay was applied to measure the levels of Th1, Th2, and Th17 cytokines and tumor necrosis factor α(TNF-α) in cell supernatant.<br /><br /><strong>RESULTS: </strong>Compared with the control group, the 0.32-and 0.64-mmol/L exposure groups had significant decreases in cell viability (P<0.05); all exposure groups had reductions in interleukin(IL)-2 and IL-12, but no significant changes in interferon-γ and IL-10. In the 1.20-mmol/L exposure group, there was an increase in IL-4, with the increasing exposure dose, IL-5 and IL-6 tended to increase first and then decrease, and there was no significant change in IL-13; with the increasing exposure dose, IL-8 tended to increase first and then decrease, and there was no significant change in IL-17. In all the exposure groups, TNF-α increased and tended to increase significantly with the increasing exposure dose(P<0.05).<br /><br /><strong>CONCLUSION: </strong>Formaldehyde exposure can cause imbalance between Th1 and Th2 cytokines secreted by 16HBE cells, as well as increased expression of IL-8 and TNF-α. |
| Doi | 10.3760/cma.j.issn.1001-9391.2016.01.006 |
| Pmid | 27014812 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Chinese |