Mast cells infiltration and decreased E-cadherin expression in ketamine-induced cystitis
Li, M; Yang, K; Wang, X; Xu, X; Zhu, L; Wang, H
| HERO ID | 4473543 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Mast cells infiltration and decreased E-cadherin expression in ketamine-induced cystitis |
| Authors | Li, M; Yang, K; Wang, X; Xu, X; Zhu, L; Wang, H |
| Journal | Toxicology Reports |
| Volume | 2 |
| Page Numbers | 205-209 |
| Abstract | <strong>BACKGROUND: </strong>Ketamine is a controlled substance and often illegally used as a recreational drug primarily by young adults. Increasing ketamine abusers associated with lower urinary tract symptoms have been reported at hospitals in recent years. Here we used a murine model to explore the changes of bladder in order to elucidate its pathogenesis.<br /><br /><strong>METHODS: </strong>ICR mice were randomly distributed into control and ketamine groups and received daily intraperitoneal injection of saline and ketamine (30 mg/kg), respectively. The bladders were excised and processed for histology at 4, 8 and 12 weeks. Tryptase and E-cadherin were investigated by immunohistochemistry in bladder tissues from ketamine-treated and control mice to assess the mast cell activation and junction protein expression.<br /><br /><strong>RESULTS: </strong>After ketamine treatment, the bladder changed to be hyperemic, inflamed, and with more fissures in mucosa. Compared with control group, the number of tryptase-positive mast cells significantly increased, which was 6.98 ± 2.89 and 23.00 ± 6.48 cells per field (100×) at 8 and 12 weeks, respectively (P = 0.016 and P = 0.003, respectively). Additionally, the expression of E-cadherin in ketamine-treated mice bladder tissue was significantly lower than that in the control tissues, P < 0.001.<br /><br /><strong>CONCLUSIONS: </strong>Increased mast cells in bladder wall and downregulated expression of E-cadherin junction protein in epithelial cells were probably associated with interstitial inflammation and fissures in mucosa. It implied that ketamine induced an interstitial cystitis. |
| Doi | 10.1016/j.toxrep.2014.12.003 |
| Pmid | 28962353 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |