Ultraviolet radiation exposure triggers neurokinin-1 receptor upregulation in ocular tissues in vivo
Gross, J; Wegener, AR; Kronschlaeger, M; Holz, FG; Schönfeld, CL; Meyer, LM
| HERO ID | 4467915 |
|---|---|
| In Press | No |
| Year | 2018 |
| Title | Ultraviolet radiation exposure triggers neurokinin-1 receptor upregulation in ocular tissues in vivo |
| Authors | Gross, J; Wegener, AR; Kronschlaeger, M; Holz, FG; Schönfeld, CL; Meyer, LM |
| Journal | Experimental Eye Research |
| Volume | 174 |
| Page Numbers | 70-79 |
| Abstract | The purpose of this study was to investigate the neurokinin receptor-1 (NKR-1) protein expression in ocular tissues before and after supra-cataract threshold ultraviolet radiation (UVR-B peak at 312 nm) exposure in vivo in a mouse model. Six-week-old C57Bl/6 mice were unilaterally exposed to a single (2.9 kJ/m2) and an above 3-fold UVR-B cataract threshold dose (9.4 kJ/m2) of UVR. UVR-exposure (λpeak = 312 nm) was performed in mydriasis using a Bio-Spectra exposure system. After latency periods of 3 and 7 days, eyes were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned and stained with fluorescence coupled antibody for NKR-1 and DAPI for cell nuclei staining. Control animals received only anesthesia but no UVR-exposure. Cataract development was documented with a Leica dark-field microscope and quantified as integrated optical density (IOD). NKR-1 is ubiquitously present in ocular tissues. An above 3-fold cataract threshold dose of UV-radiation induced NKR-1 upregulation after days 3 and 7 in the epithelium and endothelium of the cornea, the endothelial cells of the iris vessels, the pigmented epithelium/stroma of the ciliary body, the lens epithelium, pronounced in the nuclear bow region and the inner plexiform layer of the retina. A significant upregulation of NKR-1 could not be provoked with a single cataract threshold dose (2.9 kJ/m2 UVR-B) ultraviolet irradiation. All exposed eyes developed anterior subcapsular cataracts. Neurokinin-1 receptor is present ubiquitously in ocular tissues including the lens epithelium and the nuclear bow region of the lens. UV-radiation exposure to an above 3-fold UVR-B cataract threshold dose triggers NKR-1 upregulation in the eye in vivo. The involvement of inflammation in ultraviolet radiation induced cataract and the role of neuroinflammatory peptides such as substance P and its receptor, NKR-1, might have been underestimated to date. |
| Doi | 10.1016/j.exer.2018.04.016 |
| Pmid | 29705099 |
| Wosid | WOS:000444931100007 |
| Url | http://www.sciencedirect.com/science/article/pii/S0014483517305572 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Cataract; Ultraviolet radiation B; Substance P; Neurokinin receptor 1; Inflammation |