Activation of neuronal Kv7/KCNQ/M-channels by the opener QO58-lysine and its anti-nociceptive effects on inflammatory pain in rodents
Teng, BC; Song, Y; Zhang, F; Ma, TY; Qi, JL; Zhang, HL; Li, G; Wang, K
| HERO ID | 4455005 |
|---|---|
| In Press | No |
| Year | 2016 |
| Title | Activation of neuronal Kv7/KCNQ/M-channels by the opener QO58-lysine and its anti-nociceptive effects on inflammatory pain in rodents |
| Authors | Teng, BC; Song, Y; Zhang, F; Ma, TY; Qi, JL; Zhang, HL; Li, G; Wang, K |
| Journal | Acta Pharmacologica Sinica |
| Volume | 37 |
| Issue | 8 |
| Page Numbers | 1054-1062 |
| Abstract | <strong>AIM: </strong>The aim of this study was to examine the activation of neuronal Kv7/KCNQ channels by a novel modified Kv7 opener QO58-lysine and to test the anti-nociceptive effects of QO58-lysine on inflammatory pain in rodent models.<br /><br /><strong>METHODS: </strong>Assays including whole-cell patch clamp recordings, HPLC, and in vivo pain behavioral evaluations were employed.<br /><br /><strong>RESULTS: </strong>QO58-lysine caused instant activation of Kv7.2/7.3 currents, and increasing the dose of QO58-lysine resulted in a dose-dependent activation of Kv7.2/Kv7.3 currents with an EC50 of 1.2±0.2 μmol/L. QO58-lysine caused a leftward shift of the voltage-dependent activation of Kv7.2/Kv7.3 to a hyperpolarized potential at V1/2=-54.4±2.5 mV from V1/2=-26.0±0.6 mV. The half-life in plasma (t1/2) was derived as 2.9, 2.7, and 3.0 h for doses of 12.5, 25, and 50 mg/kg, respectively. The absolute bioavailabilities for the three doses (12.5, 25, and 50 mg/kg) of QO58-lysine (po) were determined as 13.7%, 24.3%, and 39.3%, respectively. QO58-lysine caused a concentration-dependent reduction in the licking times during phase II pain induced by the injection of formalin into the mouse hindpaw. In the Complete Freund's adjuvant (CFA)-induced inflammatory pain model in rats, oral or intraperitoneal administration of QO58-lysine resulted in a dose-dependent increase in the paw withdrawal threshold, and the anti-nociceptive effect on mechanical allodynia could be reversed by the channel-specific blocker XE991 (3 mg/kg).<br /><br /><strong>CONCLUSION: </strong>Taken together, our findings show that a modified QO58 compound (QO58-lysine) can specifically activate Kv7.2/7.3/M-channels. Oral or intraperitoneal administration of QO58-lysine, which has improved bioavailability and a half-life of approximately 3 h in plasma, can reverse inflammatory pain in rodent animal models. |
| Doi | 10.1038/aps.2016.33 |
| Pmid | 27264315 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |