Mipu1, a novel direct target gene, is involved in hypoxia inducible factor 1-mediated cytoprotection
Wang, K; Lei, J; Zou, J; Xiao, H; Chen, A; Liu, X; Liu, Y; Jiang, L; Xiao, Z; Xiao, X
| HERO ID | 4328909 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Mipu1, a novel direct target gene, is involved in hypoxia inducible factor 1-mediated cytoprotection |
| Authors | Wang, K; Lei, J; Zou, J; Xiao, H; Chen, A; Liu, X; Liu, Y; Jiang, L; Xiao, Z; Xiao, X |
| Journal | PLoS ONE |
| Volume | 8 |
| Issue | 12 |
| Page Numbers | e82827 |
| Abstract | Mipu1 (myocardial ischemic preconditioning up-regulated protein 1), recently identified in our lab, is a novel zinc-finger transcription factor which is up-regulated during ischemic preconditioning. However, it is not clear what transcription factor contributes to its inducible expression. In the present study, we reported that HIF-1 regulates the inducible expression of Mipu1 which is involved in the cytoprotection of HIF-1α against oxidative stress by inhibiting Bax expression. Our results showed that the inducible expression of Mipu1 was associated with the expression and activation of transcription factor HIF-1 as indicated by cobalt chloride (CoCl2) treatment, HIF-1α overexpression and knockdown assays. EMSA and luciferase reporter gene assays showed that HIF-1α bound to the hypoxia response element (HRE) within Mipu1 promoter region and promoted its transcription. Moreover, our results revealed that Mipu1 inhibited the expression of Bax, an important pro-apoptosis protein associated with the intrinsic pathway of apoptosis, elevating the cytoprotection of HIF-1 against hydrogen peroxide (H2O2)-mediated injury in H9C2 cells. Our findings implied that Bax may be a potential target gene of transcription factor Mipu1, and provided a novel insight for understanding the cytoprotection of HIF-1 and new clues for further elucidating the mechanisms by which Mipu1 protects cell against pathological stress. |
| Doi | 10.1371/journal.pone.0082827 |
| Pmid | 24349374 |
| Wosid | WOS:000328730300109 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |