Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering withEscherichia coliMetal Homeostasis
Yoon, Y; Kang, Y; Lee, W; Oh, KC; Jang, G; Kim, BG
| HERO ID | 4242101 |
|---|---|
| In Press | No |
| Year | 2018 |
| Title | Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering withEscherichia coliMetal Homeostasis |
| Authors | Yoon, Y; Kang, Y; Lee, W; Oh, KC; Jang, G; Kim, BG |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 28 |
| Issue | 2 |
| Page Numbers | 323-329 |
| Abstract | InEscherichia coli, the transcription of genes related to metal homeostasis is activated by the presence of target metals. The promoter regions of those genes can be fused with reporter genes to generate whole-cell bioreporters (WCBs); these organisms sense the presence of target metals through reporter gene expression. However, the limited number of available promoters for sensing domains restricts the number of WCB targets. In this study, we have demonstrated an alternative method to generate novel WCBs, based on the notion that since the sensing mechanisms of WCBs are related to metal transportation systems, their properties can be modulated by disrupting metal homeostasis. MutantE. colistrains were generated by deleting theznt-operon geneszntA, which encodes a zinc-export protein, andzntR, which encodes aznt-operon regulatory protein, to investigate the effects on the metal-sensing properties of WCBs. Deletion ofzntAincreased the sensitivity but abolished the selectivity of cadmium-sensing WCBs, whereas arsenic-sensing WCBs gained sensitivity toward cadmium. WhenzntRwas deleted, cadmium-sensing WCBs lost the ability to detect cadmium, and this was recovered by introducing exogenouszntR. In addition, the metal-binding site of ZntR was genetically engineered to modulate metal selectivity. This study provides a valuable platform for the development of novelE. coli-based WCBs. |
| Doi | 10.4014/jmb.1710.10012 |
| Pmid | 29212299 |
| Wosid | WOS:000426920600018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |