Experimental studies on tumor promotion by nitrogen dioxide
Ichinose, T; Fujii, K; Sagai, M
HERO ID
37661
Reference Type
Journal Article
Year
1991
Language
English
PMID
| HERO ID | 37661 |
|---|---|
| In Press | No |
| Year | 1991 |
| Title | Experimental studies on tumor promotion by nitrogen dioxide |
| Authors | Ichinose, T; Fujii, K; Sagai, M |
| Journal | Toxicology |
| Volume | 67 |
| Issue | 2 |
| Page Numbers | 211-225 |
| Abstract | The effects of nitrogen dioxide (NO2) on promotion of lung tumorigenesis induced by N-bis(2-hydroxypropyl) nitrosamine (BHPN) were investigated in male Wistar rats. In a preliminary study, the highest non-effective dose of BHPN was found to be 0.5 g per kg body weight. Rats were given a single intraperitoneal injection of BHPN at a dose of 0.5 g per kg body weight or saline at 6 weeks of age, and then exposed to clean air, 0.04 m, 0.4 ppm or 4 ppm of NO2 for 17 months, respectively. The incidence of pulmonary tumors in rats exposed to BHPN plus 4 ppm of NO2 was 12.5%; the tumors were adenomas and adenocarcinomas. Adenomas were found in 4 out of 40 rats (10%) and adenocarcinomas were found in 1 out of 40 rats (2.5%). The tumor incidence in the lungs of rats kept in BHPN plus clean air and BHPN plus 0.04 ppm of NO2 was 2.5% (1/40). In both groups adenomas were found. There was no significant difference in tumor incidence between animals exposed to BHPN plus clean air and to BHPN plus 4 ppm of NO2. No lung tumors were found in the group of BHPN plus 0.4 ppm NO2 and in animals exposed to NO2 without BHPN treatment. A high incidence of alveolar cell hyperplasia was observed in the lungs of rats injected with BHPN, and the effect of NO2 on development of alveolar cell hyperplasia was slight. On the other hand, marked bronchiolar mucosal hyperplasia was found in 17 out of 40 rats (42.5%) in the group of BHPN plus 4 ppm of NO2, and in 1 out of 40 rats (2.5%) in each of the group exposed to clean air, 0.04 ppm or 0.4 ppm of NO2 with BHPN treatment, respectively. The hyperplasia in lungs of rats exposed to 4 ppm of NO2 without BHPN treatment was slighter than that in lung of rat exposed to 4 ppm of NO2 with BHPN treatment. On the other hand, tumor incidence in the nasal cavity of rats in each of group exposed to clean air and NO2 with BHPN treatment was 97-100%. Incidence of tumors in other organs in the groups exposed to clean air and NO2 with and without BHPN treatment was very low, and NO2 had no effect on tumor development in the nasal cavity and other organs whether animals were treated with BHPN or not. Tumors found in the lungs were not metastases of tumors that had developed in the nasal cavity or in other organs. These results suggest that NO2 can promote the development of lung tumors initiated by BHPN, the NO2 concentration which has promoting activity is 4 ppm or higher. |
| Doi | 10.1016/0300-483X(91)90144-P |
| Pmid | 2031254 |
| Wosid | WOS:A1991FL84700008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | No |
| Language Text | English |
| Keyword | TUMOR PROMOTION; LUNG; NASAL CAVITY; RATS; NITROGEN DIOXIDE; N-BIS(2-HYDROXYPROPYL) NITROSAMINE (BHPN);LIPID-PEROXIDATION; RAT LUNGS; MOUSE LUNG; BUTYLATED HYDROXYTOLUENE; PROTECTIVE SYSTEMS; HYDROGEN-PEROXIDE; CHRONIC EXPOSURE; MICE; RADICALS; SMOKING |
| Is Peer Review | No |
| Is Qa | No |
| Relationship(s) |
|