Potency of N-methyl-2-pyrrolidone main metabolites to induce developmental toxic effects in rats

Saillenfait, AM; Sabate, JP; Gallissot, F

HERO ID

3564264

Reference Type

Journal Article

Subtype

Abstract

Year

2006

Language

English

HERO ID 3564264
Material Type Abstract
In Press No
Year 2006
Title Potency of N-methyl-2-pyrrolidone main metabolites to induce developmental toxic effects in rats
Authors Saillenfait, AM; Sabate, JP; Gallissot, F
Journal Reproductive Toxicology
Volume 22
Issue 2
Page Numbers 282
Abstract N-methyl-2-pyrrolidone (NMP) is a widely used industrial solvent. It was shown to be embryolethal and teratogenic when given to rats, by oral route, at doses of 500 mg/kg and greater. The aim of this study was to determine the role of NMP major metabolites in NMP-induced developmental toxic effects. Sprague–Dawley rats (20–24 per group) were given with 5-hydroxy-N-methylpyrolidone (5-HNMP), N-methylsuccinimide (NMS), or 2-hydroxy-N-methylsuccinimide (2-HNMS), by gavage, on Days 6–20 of gestation. The doses tested were: 0, 250, 500, 750 and 1000 mg/kg for 5-HNMP; 0, 500, 750, 1000, and 1250 mg/kg for NMS; and 0, 250, 500, 1000, and 1500 mg/kg for 2-HNMS. The dams were euthanized on Day 21 of gestation, and the fetuses were submitted to external examination. No sign of maternal or developmental toxicity was noted at any dose of 5-HNMP. NMS caused a significant increase in the incidence of resorptions and malformations (essentially anasarca) at 1250 mg/kg, in the presence of maternal toxicity (decreased food consumption and body weight gain). Reduced fetal weight was also found at 1000 and 1250 mg/kg. 2-HNMS did not produce adverse effect on embryo-fetal development up to the dose of 1500 mg/kg, while maternal toxicity was observed at the beginning of treatment from 500 mg/kg. Although NMS exhibited an embryotoxic potential at high doses, our results indicate that the three metabolites tested are not responsible for the prenatal toxicity of NMP.
Is Certified Translation No
Dupe Override No
Conference Location September 3-6, 2006
Conference Name 34th Annual Conference of the European Teratology Society
Conference Date Abano Terme, Padova, Italy
Is Public Yes
Language Text English
Keyword Pregnancy; Animals; Rats, Sprague-Dawley; Pyrrolidinones/TOXICITY/METABOLISM; Solvents/TOXICITY/METABOLISM; Abnormalities/ETIOLOGY; Dose-Response Relationship, Drug; NO CAS RN; 26138-58-9