Multiplex genotyping based on the melting temperature of a single locked nucleic acid probe
Ahn, JJ; Kim, JH; Kim, Y; Hong, JY; Kim, GW; Hwang, SY
| HERO ID | 3539410 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Multiplex genotyping based on the melting temperature of a single locked nucleic acid probe |
| Authors | Ahn, JJ; Kim, JH; Kim, Y; Hong, JY; Kim, GW; Hwang, SY |
| Journal | Analytical Biochemistry |
| Volume | 491 |
| Page Numbers | 72-74 |
| Abstract | Locked nucleic acid (LNA) is a modified RNA nucleotide that can be incorporated at specific positions to generate probes with the desired length, melting temperature (TM), and specificity. Here, we describe a method of multiplex genotyping based on dramatic shifts in the TM of a single dual-labeled LNA probe. Using this method, two varieties of the hairtail fish Trichiurus lepturus can be distinguished from each other, as well as from Trichiurus japonicus, based on a 1- to 2-bp difference in a fragment of mitochondrial cytochrome oxidase subunit 1. The shift in TM was 15 °C for a 1-bp mismatch and 27 °C for a 2-bp mismatch, indicating remarkable specificity. We anticipate that the method will be widely useful in applications such as species identification that require accurate, multiplex, and efficient detection of DNA polymorphisms. |
| Doi | 10.1016/j.ab.2015.09.007 |
| Pmid | 26384644 |
| Wosid | WOS:000364265200013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |