Exposure to 200 ppm of methanol increases the concentrations of interleukin-1beta and interleukin-8 in nasal secretions of healthy volunteers

Mann, WJ; Muttray, A; Schaefer, D; Klimek, L; Faas, M; Konietzko, J

HERO ID

34724

Reference Type

Journal Article

Year

2002

Language

English

PMID

12126020

HERO ID 34724
In Press No
Year 2002
Title Exposure to 200 ppm of methanol increases the concentrations of interleukin-1beta and interleukin-8 in nasal secretions of healthy volunteers
Authors Mann, WJ; Muttray, A; Schaefer, D; Klimek, L; Faas, M; Konietzko, J
Journal Annals of Otology, Rhinology, and Laryngology
Volume 111
Issue 7 Pt 1
Page Numbers 633-638
Abstract This study was designed to investigate subclinical irritating effects of methanol on functional and immunologic parameters in human respiratory epithelia. Twelve healthy, nonsmoking individuals were exposed to concentrations of 20 and 200 ppm of methanol in an exposure chamber. The concentrations of interleukin (IL)-8. IL-1"Beta", IL-6, and prostaglandin E2 (PGE2) were monitored. The saccharin transport time test was used to evaluate mucociliary transport. Video interference contrast microscopy was used to determine the ciliary beat frequency of nasal epithelial cells. Subjective symptoms were assessed wit a questionnaire. The median concentrations of IL-8 and IL-1"Beta" were significantly elevated after exposure to 200 ppm of methanol as compared to exposure to 20 ppm (IL-1"Beta", 21.4 versus 8.3 pg/mL, p = .001; IL-8. 424 versus 356 pg/mL, p = .02). The release of IL-6 and PGE2 did not change significantly (IL-6,10.3 versus 6.5 pg/mL, p = .13; PGE2, 13.6 versus 13.4 pg/mL), nor did the ciliary beat frequency or the saccharin transport time. Both IL-8 and IL-1"Beta" proved to be sensitive indicators for subclinical irritating effects of methanol in vivo. The German threshold limit of 200 ppm of methanol does not pre vent subclinical inflammatory reactions of the nasal respiratory mucosa.
Pmid 12126020
Wosid WOS:000176895200011
Is Certified Translation No
Dupe Override No
Comments ECRIB. Greek symbol is used for "beta" in title.Ann. Otol. Rhinol. Laryngol. 111: 633-638.
Is Public Yes
Language Text English
Keyword ciliary beat frequency; interleukin; methanol; mucociliary transport; nasal respiratory epithelium; prostaglandin E-2
Is Qa No