[Determination of hexabromocyclododecanes diastereoisomers in human breast milk by HPLC-MS/MS]
Tan, Z; Lu, S; Zhang, J; Jiang, Y; Zhou, J; Zhu, Z; Liu, H; Li, S; Lin, X
HERO ID
3350545
Reference Type
Journal Article
Year
2014
Language
Chinese
PMID
| HERO ID | 3350545 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | [Determination of hexabromocyclododecanes diastereoisomers in human breast milk by HPLC-MS/MS] |
| Authors | Tan, Z; Lu, S; Zhang, J; Jiang, Y; Zhou, J; Zhu, Z; Liu, H; Li, S; Lin, X |
| Journal | Weisheng Yanjiu / Journal of Hygiene Research |
| Volume | 43 |
| Issue | 5 |
| Page Numbers | 809-813 |
| Abstract | <strong>OBJECTIVE: </strong>A method for the determination of ac, 3 and γ-hexabromocyclododecanes (HBCDs) in human breast milk was developed by HPLC-MS/MS.<br /><br /><strong>METHODS: </strong>3 -5 g human breast milk powder was spiked with '3C-HBCDs and then been extracted using Soxhlet extraction. The extract was dried and dissolved in 6ml of cyclohexane/ethyl acetate (1:1 ), then purified by gel permeation chromatography (GPC). The effluent was concentrated with rotary evaporation and then re-dissolved in hexane. 2ml of sulphuric acid was added to remove the fat for further clean-up. After drying under nitrogen, the supernatant was dissolved in 100 µl of methanol and finally determined by HPLC-MS/MS.<br /><br /><strong>RESULTS: </strong>The linear range for the three diastereoisomers of HBCDs was in 1 - 50 µg/L, with correlation coefficients ranging from 0. 9997 to 0.998. The detection limits of the three diastereoisomers ranged from 0. 12 to 0. 22 µg/L. The recoveries for three spiking levels ranged from 82. 80% to 110. 60% . The intra-day and inter-day relative standard deviations (RSD) were all less than 9. 4%.<br /><br /><strong>CONCLUSION: </strong>The developed method was simple, convenient and sensitive. It was suitable for the determination of or, P3 and y-HBCDs in breast milk and other matrix in the future. |
| Pmid | 25438540 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929941866&partnerID=40&md5=2644317323bf1ad87c9dafa035b47aa7 |
| Is Public | Yes |
| Language Text | Chinese |
| Relationship(s) |
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