Toxicity of an attapulgite sample studied in vivo and in vitro
Renier, A; Fleury, J; Monchaux, G; Nebut, M; Bignon, J; Jaurand, MC
HERO ID
3085259
Reference Type
Book/Book Chapter
Year
1989
Language
English
PMID
| HERO ID | 3085259 |
|---|---|
| Year | 1989 |
| Title | Toxicity of an attapulgite sample studied in vivo and in vitro |
| Book Title | Non-occupational exposure to mineral fibres |
| Authors | Renier, A; Fleury, J; Monchaux, G; Nebut, M; Bignon, J; Jaurand, MC |
| Editor | Bignon, J; Peto, J; Saracci, R |
| Publisher Text | International Agency for Research on Cancer |
| City | Lyon, France |
| Page Numbers | 180-184 |
| Abstract | Conflicting data are found in the literature concerning the carcinogenic potency of attapulgite. We tested the carcinogenic potency of French attapulgite in rats, and compared it with 2 chrysotile samples: Rhodesian UICC (Ch A) and short Canadian fibres (Ch C). The mean length of the fibres was 0.77 micron (attapulgite), 3.21 microns (Ch A) and 1.25 microns (Ch C). The mean diameter was 0.06 micron in the 3 samples. The particles (20 mg) in saline were inoculated into the pleural cavity of Sprague-Dawley rats allowed to survive for their full lifespan. The incidence rates of mesothelioma were: 0% (saline controls), 0% (attapulgite), 19% (ChC) and 48% (Ch A). In vitro studies were carried out using cultures of rat pleural mesothelial cells (RPMC). Attapulgite and Ch C did not modify cell growth except at high doses of 10 micrograms/cm2. Unscheduled DNA synthesis (UDS) was detected using [3H]thymidine incorporation in confluent RPMC (GoG1 arrested) and a scintillation method. UDS was stimulated with either Ch A or Ch C at doses ranging from 2 to 10 micrograms/cm2. In contrast, attapulgite did not significantly enhance [3H]thymidine incorporation at doses ranging from 2 to 20 micrograms/cm2. The results show that the attapulgite tested here had no carcinogenic potency. The in vivo and in vitro reactivity of the fibres used in this experiment might perhaps be related to the fibre size; however, other parameters may also be important. |
| Pmid | 2545606 |
| Wosid | MEDLINE:2545606 |
| Is Certified Translation | No |
| Dupe Override | No |
| Series | IARC Scientific Publications, no. 90 |
| Isbn | 9789283211907 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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