Role of PTEN in TNFα induced insulin resistance
Bulger, DA; Conley, J; Conner, SH; Majumdar, G; Solomon, SS
| HERO ID | 2966228 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Role of PTEN in TNFα induced insulin resistance |
| Authors | Bulger, DA; Conley, J; Conner, SH; Majumdar, G; Solomon, SS |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 461 |
| Issue | 3 |
| Page Numbers | 533-536 |
| Abstract | <strong>AIMS/HYPOTHESIS: </strong>PTEN may play a reversible role in TNFα induced insulin resistance, which has been linked to obesity-associated insulin resistance (IR).<br /><br /><strong>METHODS: </strong>Western blots for PTEN and p-Akt were performed on H-411E liver cells incubated with insulin, TNFα, and in selected experiments VO-OHpic vanadium complex in the presence and absence of PTEN siRNA. Total PTEN was compared to β-actin loading control and p-Akt was compared to total Akt.<br /><br /><strong>RESULTS: </strong>Western blot and Real Time RT-PCR experiments showed increased PTEN after TNFα treatment (p = 0.04); slightly decreased PTEN after insulin treatment; and slightly increased PTEN after insulin + TNFα treatment. PTEN siRNA markedly inhibited the TNFα-induced increase in PTEN (p < 0.01) without significantly changing the p-Akt levels. The vanadium complex, exhibiting insulin-like effects, also significantly prevented the TNFα-induced increase in PTEN. Combining insulin and VO-OHpic was additive, providing both proof of concept and insight into mechanism.<br /><br /><strong>DISCUSSION: </strong>The PTEN increase due to TNFα treatment was reversible by both PTEN siRNA knockdown and VO-OHpic treatment. Thus, PTEN is identified as a potential new therapeutic target for reducing IR in Type 2 DM. |
| Doi | 10.1016/j.bbrc.2015.04.063 |
| Pmid | 25918024 |
| Wosid | WOS:000355157200016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |