Adipocyte membrane glycerol permeability is involved in the anti-adipogenic effect of conjugated linoleic acid
Martins, SV; Madeira, A; Lopes, PA; Pires, VM; Alfaia, CM; Prates, JA; Moura, T; Soveral, G
HERO ID
2902237
Reference Type
Journal Article
Year
2015
Language
English
PMID
| HERO ID | 2902237 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Adipocyte membrane glycerol permeability is involved in the anti-adipogenic effect of conjugated linoleic acid |
| Authors | Martins, SV; Madeira, A; Lopes, PA; Pires, VM; Alfaia, CM; Prates, JA; Moura, T; Soveral, G |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 458 |
| Issue | 2 |
| Page Numbers | 356-361 |
| Abstract | Conjugated linoleic acid (CLA), a group of minor fatty acids from ruminant origin, has long been recognized as a body fat lowering agent. Given the trans(t)10,cis(c)12-CLA well documented interference on lipolysis, we hypothesized for adipocytes altered permeation to glycerol when supplemented with this isomer. 3T3-L1 murine differentiated adipocytes were medium supplemented with linoleic acid (LA) and individual or combined c9,t11 and t10,c12-CLA isomers. Adipocytes treated with the t10,c12-CLA isomer and CLA mixture showed reduced triacylglycerols content (p < 0.001), re-enforcing the t10,c12-CLA as the anti-adipogenic CLA isomer. This finding was supported by decreased Δ9-desaturase index and adipocyte differentiation markers for the t10,c12-CLA group (p < 0.001), which suggest reduced lipogenesis and differentiation, respectively. The glycerol permeability was higher in all CLA treated cells compared to control and LA groups (p < 0.05). The increase in glycerol permeability agrees with both reduced triacylglycerols and non-osmotic cellular volume in the t10,c12-CLA and CLA mixture groups. Taken together, our data suggest that the increased adipocyte plasma membrane glycerol fluxes may be part of the anti-adipogenic response to CLA treatments. |
| Doi | 10.1016/j.bbrc.2015.01.116 |
| Pmid | 25646690 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |