Heat differentiated complement factor profiling
Hamsten, C; Skattum, L; Truedsson, L; von Döbeln, U; Uhlén, M; Schwenk, JM; Hammarström, L; Nilsson, P; Neiman, M
| HERO ID | 2902046 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Heat differentiated complement factor profiling |
| Authors | Hamsten, C; Skattum, L; Truedsson, L; von Döbeln, U; Uhlén, M; Schwenk, JM; Hammarström, L; Nilsson, P; Neiman, M |
| Journal | Journal of Proteomics |
| Volume | 126 |
| Page Numbers | 155-162 |
| Abstract | Complement components and their cascade of reactions are important defense mechanisms within both innate and adaptive immunity. Many complement deficient patients still remain undiagnosed because of a lack of high throughput screening tools. Aiming towards neonatal proteome screening for immunodeficiencies, we used a multiplex profiling approach with antibody bead arrays to measure 9 complement proteins in serum and dried blood spots. Several complement components have been described as heat sensitive, thus their heat-dependent detectability was investigated. Using sera from 16 patients with complement deficiencies and 23 controls, we confirmed that the proteins C1q, C2, C3, C6, C9 and factor H were positively affected by heating, thus the identification of deficient patients was improved when preheating samples. Measurements of C7, C8 and factor I were negatively affected by heating and non-heated samples should be used in analysis of these components. In addition, a proof of concept study demonstrated the feasibility of labeling eluates from dried blood spots to perform a subsequent correct classification of C2-deficiencies. Our study demonstrates the potential of using multiplexed single binder assays for screening of complement components that open possibilities to expand such analysis to other forms of deficiencies. |
| Doi | 10.1016/j.jprot.2015.05.027 |
| Pmid | 26047714 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |