Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study
Morisco, F; Aprea, E; Lembo, V; Fogliano, V; Vitaglione, P; Mazzone, G; Cappellin, L; Gasperi, F; Masone, S; De Palma, GD; Marmo, R; Caporaso, N; Biasioli, F
HERO ID
2901747
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 2901747 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study |
| Authors | Morisco, F; Aprea, E; Lembo, V; Fogliano, V; Vitaglione, P; Mazzone, G; Cappellin, L; Gasperi, F; Masone, S; De Palma, GD; Marmo, R; Caporaso, N; Biasioli, F |
| Journal | PLoS ONE |
| Volume | 8 |
| Issue | 4 |
| Page Numbers | e59658 |
| Abstract | <strong>UNLABELLED: </strong>The aim of the present work was to test the potential of Proton Transfer Reaction Time-of-Flight Mass Spectrometry (PTR-ToF-MS) in the diagnosis of liver cirrhosis and the assessment of disease severity by direct analysis of exhaled breath. Twenty-six volunteers have been enrolled in this study: 12 patients (M/F 8/4, mean age 70.5 years, min-max 42-80 years) with liver cirrhosis of different etiologies and at different severity of disease and 14 healthy subjects (M/F 5/9, mean age 52.3 years, min-max 35-77 years). Real time breath analysis was performed on fasting subjects using a buffered end-tidal on-line sampler directly coupled to a PTR-ToF-MS. Twelve volatile organic compounds (VOCs) resulted significantly differently in cirrhotic patients (CP) compared to healthy controls (CTRL): four ketones (2-butanone, 2- or 3- pentanone, C8-ketone, C9-ketone), two terpenes (monoterpene, monoterpene related), four sulphur or nitrogen compounds (sulfoxide-compound, S-compound, NS-compound, N-compound) and two alcohols (heptadienol, methanol). Seven VOCs (2-butanone, C8-ketone, a monoterpene, 2,4-heptadienol and three compounds containing N, S or NS) resulted significantly differently in compensate cirrhotic patients (Child-Pugh A; CP-A) and decompensated cirrhotic subjects (Child-Pugh B+C; CP-B+C). ROC (Receiver Operating Characteristic) analysis was performed considering three contrast groups: CP vs CTRL, CP-A vs CTRL and CP-A vs CP-B+C. In these comparisons monoterpene and N-compound showed the best diagnostic performance.<br /><br /><strong>CONCLUSIONS: </strong>Breath analysis by PTR-ToF-MS was able to distinguish cirrhotic patients from healthy subjects and to discriminate those with well compensated liver disease from those at more advanced severity stage. A breath-print of liver cirrhosis was assessed for the first time. |
| Doi | 10.1371/journal.pone.0059658 |
| Pmid | 23573204 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |